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L3771

Sigma-Aldrich

十二烷基硫酸钠

BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)

别名:
月桂基硫酸盐 钠盐, Dodecyl sulfate 钠盐, Sodium lauryl sulfate, SDS, Dodecyl sodium sulfate
线性分子式:
CH3(CH2)11OSO3Na
CAS号:
分子量:
288.38
Beilstein:
3599286
EC 号:
MDL编号:
eCl@ss:
39093306
PubChem化学物质编号:
NACRES:
NB.22

质量水平

200

等级

for molecular biology

描述

anionic

产品线

BioReagent

测定

≥98.5% (GC)

分子量

micellar 18,000

聚集数

62

technique(s)

electrophoresis: suitable

CMC

7-10 mM (20-25°C)

mp

204-207 °C (lit.)

转变温度

cloud point >100 °C

溶解性

water: soluble

痕量阴离子

chloride (Cl-): ≤500 ppm
phosphate (PO43-): ≤10 ppm

痕量阳离子

heavy metals (as Pb): ≤10 ppm

吸光度

≤0.1 at 260 at 3%
≤0.1 at 280 at 3%

HLB

40

适用性

suitable for electrophoresis

异质活性

DNase, RNase, none detected

SMILES string

[Na+].CCCCCCCCCCCCOS([O-])(=O)=O

InChI

1S/C12H26O4S.Na/c1-2-3-4-5-6-7-8-9-10-11-12-16-17(13,14)15;/h2-12H2,1H3,(H,13,14,15);/q;+1/p-1

InChI key

DBMJMQXJHONAFJ-UHFFFAOYSA-M

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一般描述

十二烷基硫酸钠(SDS)是一种阴离子去污剂,适合用于对蛋白进行变性。它具有一个带有带负电荷的硫酸盐长链脂肪族基团。这使得SDS成为一种两亲性去污剂。

应用

用于增溶和蛋白质变性,适用于PAGE变性应用。 大多数蛋白质以1.4 g SDS/g 蛋白质的比例结合SDS。 相对于结合的SDS自带的总负电荷,蛋白质固有电荷几乎可忽略不计。 每种蛋白质的质荷比基本相同,且基于分子大小在凝胶中迁移。
十二烷基硫酸钠已用于:
  • 染色体免疫沉淀
  • SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)
  • 作为用于免疫印迹的放射免疫沉淀检测缓冲液的一种组分
阴离子洗涤剂

包装

25, 100, 500 g in poly bottle
1 kg in poly bottle

分析说明

针对用于变性聚丙烯酰胺凝胶电泳进行了测试。

警示用语:

Danger

危险分类

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 3 - Eye Dam. 1 - Flam. Sol. 2 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

4.1B - Flammable solid hazardous materials

WGK

WGK 2

闪点(F)

338.0 °F

闪点(C)

170 °C

个人防护装备

Eyeshields, Faceshields, Gloves, type P1 (EN143) respirator filter, type P3 (EN 143) respirator cartridges

分析证书

原产地证书 (CofO)

Shane Stegeman et al.
Endocrine-related cancer, 22(2), 265-276 (2015-02-12)
The oncogene MDM4, also known as MDMX or HDMX, contributes to cancer susceptibility and progression through its capacity to negatively regulate a range of genes with tumour-suppressive functions. As part of a recent genome-wide association study it was determined that
Kazuya Ishikawa et al.
Nature communications, 5, 5430-5430 (2014-11-13)
Pathogen effector proteins are delivered to host cells to suppress plant immunity. However, the mechanisms by which effector proteins function are largely unknown. Here we show that expression of XopP(Xoo), an effector of rice pathogen Xanthomonas oryzae pv. oryzae, in
S Bavamian et al.
Molecular psychiatry, 20(5), 573-584 (2015-01-28)
Bipolar disorder (BD) is a heritable neuropsychiatric disorder with largely unknown pathogenesis. Given their prominent role in brain function and disease, we hypothesized that microRNAs (miRNAs) might be of importance for BD. Here we show that levels of miR-34a, which
Hannah M Kaneb et al.
Human molecular genetics, 24(5), 1363-1373 (2014-10-26)
Amyotrophic lateral sclerosis (ALS) is a fatal neurodegenerative disorder characterized by the selective death of motor neurons. Causative mutations in the global RNA-processing proteins TDP-43 and FUS among others, as well as their aggregation in ALS patients, have identified defects
Satoshi Fukuyama et al.
Nature communications, 6, 6600-6600 (2015-03-26)
Seasonal influenza A viruses cause annual epidemics of respiratory disease; highly pathogenic avian H5N1 and the recently emerged H7N9 viruses cause severe infections in humans, often with fatal outcomes. Although numerous studies have addressed the pathogenicity of influenza viruses, influenza

实验方案

Plasmid DNA Preparation

Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.

Stripping and Reprobing Western Blotting Membranes

This page shows and discusses three protocols for stripping and reprobing a western blot membrane.

使用TurboMix™ Bis-Tris制胶试剂盒手动灌制PAGE和SDS-PAGE凝胶

聚丙烯酰胺凝胶概述以及使用TurboMix™ Bis-Tris制胶试剂盒手动灌注聚丙烯酰胺凝胶的详细说明

Hand-casting gels for PAGE and SDS-PAGE using TurboMix™ Bis-Tris Gel Casting Kits

Overview of polyacrylamide gel chemistry and detailed instructions for hand-casting polyacrylamide gels using TurboMix™ Bis-Tris Gel Casting Kits

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Lysis & Protein Extraction

An overview of cell lysis and protein extraction methods including detergent solubilization, freeze-thaw lysis, osmotic shock, sonication, enzymatic cell lysis, and mechanical disruption techniques such as Dounce, Polytron, and mortar and pestle homogenization.

裂解和蛋白提取

常用的细胞裂解和蛋白质提取方法包括表面活性剂溶解法、冻融裂解法、渗透压休克法、超声破碎法、酶解细胞裂解法以及机械破碎技术(如Dounce、Polytron、研钵和研棒均质化)。

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

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