Merck

L4895

Sigma-Aldrich

来自 普通小麦 的凝集素(小麦)

FITC conjugate, lyophilized powder

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别名:
WGA, Wheat germ agglutinin
NACRES:
NA.32

偶联物

FITC conjugate

质量水平

形式

lyophilized powder

效能

<50 μg/mL agglutination activity

组成

Protein, ~50% Lowry

标记范围

~2 mol FITC per mol protein

储存温度

2-8°C

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一般描述

凝集素是碳水化合物结合蛋白。它们无处不在,常见于真菌、植物和动物体内。该蛋白质的二级结构富含β-链,并且在表面上具有碳水化合物结合位点。

应用

Triticum vulgaris(小麦)来源凝集素已用于:
  • 处理转染细胞,以进行免疫荧光分析
  • 立体定位注射到小鼠体内,以进行神经元示踪
  • 免疫组化,以分析心肌细胞

用于荧光检测含有 β(1→4)-N-乙酰基-D葡糖胺的糖蛋白

生化/生理作用

凝集素在糖缀合物鉴定、成像和靶向过程中发挥着关键作用。它可用于芯片分析,实现有效的糖组图谱分析。在植物和真菌体内,凝集素可防御病原体/寄生物。凝集素在宿主识别和组织粘附中同样发挥主要作用。
WGA 不具有血型特异性,但具有与 N-乙酰基-β-D-葡糖胺残基和 N-乙酰基-β-D-葡糖胺低聚物的亲和性。WGA 不含蛋白结合糖。

包装

基于蛋白含量进行包装。

外形

含有磷酸盐缓冲盐

制备说明

由亲和纯化凝集素制备偶联物。

相关产品

产品编号
说明
价格

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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Sarah Aileen Lewis et al.
PloS one, 13(8), e0201986-e0201986 (2018-08-14)
Tissues with high-energy demand including the heart are rich in the energy-producing organelles, mitochondria, and sensitive to mitochondrial dysfunction. While alterations in mitochondrial function are increasingly recognized in cardiovascular diseases, the molecular mechanisms through which changes in mitochondria lead to
Yihua Bei et al.
Journal of cellular and molecular medicine, 21(8), 1648-1655 (2017-03-18)
The adult heart retains a limited ability to regenerate in response to injury. Although exercise can reduce cardiac ischaemia/reperfusion (I/R) injury, the relative contribution of cardiac cell proliferation including newly formed cardiomyocytes remains unclear. A 4-week swimming murine model was
Hai Li et al.
Langmuir : the ACS journal of surfaces and colloids, 26(8), 5603-5609 (2009-12-02)
The technique to pattern aminosilanes on hydroxyl-terminated substrates will open up extensive applications in many fields. There are some existing methods to pattern aminosilanes, in particular, (3-aminopropyl)triethoxysilane (APTES) on SiO(2) and glass substrates through indirect routes. However, few reports focus
Chronic effects of mild hyperglycaemia on left ventricle transcriptional profile and structural remodelling in the spontaneously type 2 diabetic Goto-Kakizaki rat
Dsouza A, et al.
Heart Failure Reviews, 19(1), 65-74 (2014)
Midbrain dopamine neurons associated with reward processing innervate the neurogenic subventricular zone
Lennington JB, et al.
The Journal of Neuroscience, 31(37), 13078-13087 (2011)

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