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L4919

Sigma-Aldrich

溶菌酶 来源于鸡蛋白

BioUltra, lyophilized powder, ≥98% (SDS-PAGE), ≥40,000 units/mg protein

别名:
胞壁质酶, 粘肽 N-乙酰胞壁质水解酶
CAS号:
EC 号:
MDL编号:

质量水平

200

等级

for molecular biology

产品线

BioUltra

测定

≥98% (SDS-PAGE)

形式

lyophilized powder

specific activity

≥40,000 units/mg protein

分子量

single-chain 14.3 kDa

组成

Protein, ≥90%

储存温度

−20°C

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一般描述

溶菌酶广泛存在于动植物界。它是天然的食品防腐剂,也存在于特定的细菌细胞壁中。溶菌酶通常存在于眼泪、牛奶、尿液和唾液中。

应用

溶菌酶(来自鸡蛋白)已用于:
  • 蛋白动力学的介电谱研究
  • 作为人溶菌酶活性检测对照
  • 作为护理隐形眼镜的浸泡液的添加剂

酶分解细菌的细胞壁;用于制备原生质球。

包装

500 mg in poly bottle
1, 5 g in poly bottle

生化/生理作用

溶菌酶参与防御机制。它具有通过在底物中产生空间应力刺激催化的能力。
溶菌酶水解肽聚糖中 N-乙酰胞壁酸和 N-乙酰基-D-葡糖胺残基间的 β(1→4) 键以及壳糊精中 N-乙酰基-D-葡糖胺残基间的 β(1→4)键。革兰氏阳性细胞对该水解作用非常敏感,因为它们的细胞壁含有较高比例的肽聚糖。由于存在外膜以及比例较低的肽聚糖,革兰氏阴性细菌对该水解作用不太敏感。然而,细菌外膜中如果存在与金属离子螯合的 EDTA,那么这些细胞也可能被水解。

该酶在较宽的 pH 范围(6.0 到 9.0)内具有活性。与 pH 为 9.2 时相比,pH 为 6.2 时可在更广的离子强度(0.02 到 0.100M)范围内观察到最大活性。

特点和优势

  • 通过反复结晶和透析进行高度纯化
  • 每个批次均对从 E. coli中分离质粒DNA进行测试

单位定义

在25℃、pH 6.24条件下,以溶壁微球菌悬浮液为底物,一个单位在2.6 mL反应混合物中可产生0.001/min的ΔA450 (光程为1 cm)。

制备说明

3 次结晶

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK Germany

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

分析证书

原产地证书 (CofO)

Protein dynamics in a broad frequency range: Dielectric spectroscopy studies
Nakanishi M and Sokolov AP
Journal of Non-Crystalline Solids, 407, 478-485 (2015)
Natural Food Antimicrobial Systems (2000)
Metabolic engineering of probiotic Saccharomyces boulardii
Liu JJ, et al.
Applied and Environmental Microbiology, 82(8), 2280-2287 (2016)
Corneal cell adhesion to contact lens hydrogel materials enhanced via tear film protein deposition
Elkins CM, et al.
Testing, e105512-e105512 (2014)
Jordi van Gestel et al.
Nature ecology & evolution, 3(8), 1184-1196 (2019-07-25)
Microbes are exposed to changing environments, to which they can respond by adopting various lifestyles such as swimming, colony formation or dormancy. These lifestyles are often studied in isolation, thereby giving a fragmented view of the life cycle as a...

技术文章

HPLC Analysis of Proteins on TSKgel® BioAssist S

Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis; Lysozyme from chicken egg white, lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

HPLC Analysis of Proteins on Proteomix® SCX-NP5

Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis; Lysozyme from chicken egg white, lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

HPLC Analysis of Proteins by Cation Exchange on Proteomix® WCX-NP5: Affect of pH

Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis; Lysozyme from chicken egg white, lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

HPLC Analysis of Proteins by Cation Exchange on Proteomix® WCX: Affect of Particle Size

Separation of Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein; α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder; Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis; Lysozyme from chicken egg white, lyophilized powder, protein ≥90 %, ≥40,000 units/mg protein

查看所有结果

实验方案

Enzymatic Assay of Lysozyme (EC 3.2.1.17)

This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.

GenElute™ Bacterial Genomic DNA Kit Protocol (NA2100, NA2110, NA2120)

GenElute™ Bacterial Genomic DNA Kit protocol describes a simple and convenient way for the isolation of pure genomic DNA from bacteria.

相关内容

溶菌酶的酶学测定(EC 3.2.1.17)

该酶速率测定可用于溶菌酶产物。它不适用于在测定在琼脂糖上的重组型或不溶性溶菌酶。

GenElute™细菌基因组DNA试剂盒实验方案(NA2100、NA2110、NA2120)

的GenElute™细菌基因组DNA试剂盒提供了一种从细菌中分离纯基因组DNA的简便方法。

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