Merck

L6533

Sigma-Aldrich

来自麦胚(硬皮豆)的凝集素

biotin conjugate, buffered aqueous solution

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别名:
麦胚凝集素, DBA
NACRES:
NA.32

偶联物

biotin conjugate

质量水平

形式

buffered aqueous solution

组成

Protein, ≥2.5 mg/mL biuret

标记范围

≥3 mol biotin per mol lectin

储存温度

−20°C

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此商品
L1882L9017L5142
storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

200

conjugate

biotin conjugate

conjugate

agarose conjugate

conjugate

-

conjugate

biotin conjugate

composition

Protein, ≥2.5 mg/mL biuret

composition

-

composition

-

composition

Protein, ≥80% E1%/280

extent of labeling

≥3 mol biotin per mol lectin

extent of labeling

5-10 mg per mL

extent of labeling

-

extent of labeling

2-4 mol biotin per mol protein

一般描述

来自 双花扁豆(硬皮豆)的凝集素是一种异源四聚体糖蛋白,其分子量约为113 kDa。双花扁豆凝集素(DBA)对于末端α连接的N-乙酰基-D-半乳糖胺(GalNAc)有特异性。它是一种膳食凝集素。

应用

来自双花扁豆(硬皮豆)的凝集素已被用于:
  • 表征小鼠子宫自然杀伤(uNK)细胞
  • 采用凝集素双重染色定位核因子-κB(NF-κB)活化
  • 作为物种特异性生殖母细胞标记物用于确定供体细胞定植
  • 标记未分化的精原细胞/猫睾丸组织

生化/生理作用

双花扁豆凝集素(DBA)具有结合活性,可用作双核细胞的标记物,并可指示其分化状态。它可用作原始生殖细胞(PGC)、生殖母细胞、前精原细胞和精原细胞的标记物。
DBA具有抗人-A1血型特异性,可用于区分A1和A2血型。 DBA具有很强的抗-Cad活性,并且对末端N-乙酰基-α-D-半乳糖胺基残基具有亲和力。从亲和纯化的凝集素制备偶联物。

包装

按活性结合物毫克数出售。

外形

pH 7.5,含 10 mM HEPES、0.15 M NaCl、0.08% 叠氮化钠、0.1 mM Ca2+

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable


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Spermatogonial stem cell organization in felid testis as revealed by Dolichos biflorus lectin
Escada-Rebelo S, et al.
Andrology, 4(6), 1159-1168 (2016)
Association of Dolichos biflorus lectin binding with full differentiation of bovine trophoblast cells
Nakano H, et al.
Reproduction (Cambridge, England), 124(4), 581-592 (2002)
Rajesh Dash et al.
JACC. Cardiovascular interventions, 11(2), 195-205 (2018-01-20)
The study investigated whether a dose response exists between myocardial salvage and the depth of therapeutic hypothermia. Cardiac protection from mild hypothermia during acute myocardial infarction (AMI) has yielded equivocal clinical trial results. Rapid, deeper hypothermia may improve myocardial salvage.
B Anne Croy et al.
Methods in molecular biology (Clifton, N.J.), 612, 465-503 (2009-12-25)
The term uterine natural killer (uNK) cell is applied in mice to an abundant but transient NK cell population that undergoes unique, terminal differentiation within embryo implantation sites during endometrial decidualization and pregnancy. In mice, decidualization is induced by attachment
Tubular NF-kB and AP-1 activation in human proteinuric renal disease
Mezzano SA, et al.
Kidney International, 60(4), 1366-1377 (2001)

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