Merck
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L7543

Sigma-Aldrich

抗-LC3B 兔抗

enhanced validation

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

Synonym(s):
LC3B抗体 - 产自兔中的抗-LC3B抗体, Lc3B抗体, Sigma Lc3抗体, 抗-MAP1A/1B轻链3 B, 抗-MAP1A/1B轻链3样蛋白2, 抗-MAP1A/MAP1B LC3 B, 抗-微管相关蛋白1A/B轻链3B前体, 抗-自噬相关泛素样修饰物LC3 B, 抗-自噬相关蛋白LC3 B
MDL编号:
NACRES:
NA.41

生物来源

rabbit

偶联物

unconjugated

抗体形式

affinity isolated antibody

antibody product type

primary antibodies

克隆

polyclonal

形式

buffered aqueous solution

分子量

antigen 16-18 kDa

species reactivity

mouse, human, rat

增强验证

functional assay
Learn more about Antibody Enhanced Validation

浓度

~1 mg/mL

technique(s)

indirect immunofluorescence: 5-10 μg/mL using human HeLa cells
western blot: 1-2 μg/mL using whole extracts of rat and mouse brain

UniProt登记号

application(s)

research pathology

运输

dry ice

储存温度

−20°C

Gene Information

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Anti-LC3B antibody produced in rabbit ~1 mg/mL, affinity isolated antibody, buffered aqueous solution

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Anti-LC3A antibody produced in rabbit ~1 mg/mL, affinity isolated antibody, buffered aqueous solution

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抗LC3A 兔抗

biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

form

buffered aqueous solution

form

buffered aqueous glycerol solution

form

buffered aqueous solution

form

buffered aqueous solution

mol wt

antigen 16-18 kDa

mol wt

-

mol wt

~16/18 kDa

mol wt

antigen 16-18 kDa

一般描述

MAP1LC3B基因(微管相关蛋白1轻链3β)是酵母中的自噬相关基因ATG8的一种同源基因。所编码的蛋白被称为pro-MAP1LC3B,是一种由ATG4切割以产生被称为MAP1LC3B-I的胞质形式的前体形式。
微管相关蛋白1轻链3 (MAPLC3/LC3)首先在大鼠中发现,是一种与大鼠脑中微管相关蛋白1A和1B共纯化的蛋白。Lc3以两种形式存在于细胞中。一种是LC3-I (18 kDa)细胞质,另一种是与自噬体膜相关的LC3-II (16 kDa)。微管相关蛋白轻链 3-I(LC3-I)被哺乳动物自噬相关 7(Atg7)和 Atg3 修饰为膜结合形式 LC3-II(LC3-磷脂缀合物),分别为 E1- 和 E2- 样酶。现已鉴定出大鼠LC3的三个人类直系同源基因,分别命名为MAP1LC3A/LC3AA,MAP1LC3B/LC3B和MAP1LC3C/ LC3C。人类蛋白质表现出两种形式,分别代表胞质(I型)和膜结合(II型)形式。这三种人类同源基因在人类组织中呈现了不同的表达模式。

特异性

通过免疫印迹,抗体可识别人、大鼠和小鼠LC3B-1和LC3B-II(分别为~18 kDa和~16 kDa)。

免疫原

对应于人LC3B第2-15个氨基酸的合成肽,通过C-末端的半胱氨酸残基与KLH发生偶联。相应的序列在大鼠和小鼠中相差一个氨基酸。

应用

产自兔中的抗-LC3B抗体已用于western blotting和免疫组化。
产自兔中的抗-LC3B抗体已用于蛋白质印迹和免疫组化。

生化/生理作用

在自噬过程中被插入至生长中的自噬囊泡的内膜和外膜前,MAP1LC3B-I(微管相关蛋白1轻链3β-I)会与MAP1LC3B-II发生偶联。MAP1LC3B的细胞分布和加工有助于对自噬进行监测。缺乏MAP1LC3B的细胞被发现在自噬囊泡的形成中存在缺陷,其改变的表型与自噬在能量稳态和蛋白聚集体去除中的作用相关。它可作为一种自噬的标志物。
微管相关蛋白1轻链3 (MAPLC3/LC3)的表达水平与自噬小体形成程度相关。LC3B可用作自噬体标记物。

外形

含有15 mM叠氮化钠的pH 7.4 0.01 M磷酸盐缓冲液中的溶液。

免责声明

除非在我们的产品目录或产品随附的其他公司文档中另有说明,我们的产品仅供研究使用并不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途、任何类型的食用或应用于人或动物。

储存分类代码

10 - Combustible liquids

WGK

WGK 1

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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