Merck

L8777

Sigma-Aldrich

来自美洲商陆(商陆)的凝集素

lyophilized powder, BioReagent, suitable for cell culture

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别名:
PWM, 美洲商陆有丝分裂原
MDL编号:
NACRES:
NA.32

无菌性

aseptically filled

质量水平

产品线

BioReagent

形式

lyophilized powder

效能

≤0.3 μg/mL mitogenic activity

technique(s)

cell culture | mammalian: suitable

溶解性

PBS: 1 mg/mL

储存温度

2-8°C

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此商品
L9379L4144L1668
potency

≤0.3 μg/mL mitogenic activity

potency

≤0.3 μg/mL mitogenic activity

potency

erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at 250 μg lectin/ml.), ≤1.0 μg per mL leucoagglutination activity, ≤5 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells)

potency

≤10 μg per mL mitogenic activity (Bromdeoxyuridine incorporation in human peripheral blood mononuclear cells)

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

solubility

PBS: 1 mg/mL

solubility

-

solubility

DPBS: 5 mg/mL, slightly hazy, colorless

solubility

PBS: 5 mg/mL, clear to slightly hazy, colorless to faintly yellow (pH 6.8)

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

一般描述

凝集素是植物来源的蛋白质,其具有结合某些神经元表面糖蛋白和糖脂的能力。他们的这种能力有助于它们被用作神经解剖学示踪剂,因为它们以逆行或顺行方式被采集和运输。

应用

来自美洲商陆(商陆)的凝集素用于增殖测定。(使用常规测定,通过在新合成的DNA中掺入 3H 胸苷来测量细胞增殖反应)。

生化/生理作用

凝集素是碳水化合物结合蛋白,对糖部分具有高度特异性。商陆凝集素(PL)是对含有N-乙酰氨基葡糖的糖特异的凝集素,其通过与细胞表面结合来刺激外周淋巴细胞进行有丝分裂。从商陆(美洲商陆)的根中分离出四种类型的凝集素。它们含有同源结构域,但具有不同的分子大小和生物学特性。
PWM 从商陆根中提取。该蛋白质具有红血球凝集、白血球凝集和促有丝分裂特性。PWM 不具有血型特异性,但具有与 N-乙酰基-β-D-葡糖胺低聚物的亲和性。

质量

部分纯化的 TCA 沉淀。
基本不含盐。

分析说明

通过将 BrdU 掺入 HeLa 细胞培养基中测定有丝分裂促进活性。

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1 - Skin Sens. 1

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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