M0268

Eagle最低必需培养基

With Earle′s salts and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

• Synonym(s): MEM
• NACRES: NA.75

Properties

• 质量水平: 500
• 形式: powder
• technique(s): cell culture | mammalian: suitable
• 组分: HEPES: no; phenol red: yes; Earle’s salts (5% CO₂): yes; NaHCO₃: no; L-glutamine: yes; sodium pyruvate: no
• 运输: ambient
• 储存温度: 2-8°C

Description

一般描述

Minimum Essential Medium Eagle (MEM) is one of the most widely used synthetic cell culture media. MEM has been used for culturing several cells grown in monolayers. It is commonly used to grow attached cell lines, such as fibroblasts, in the presence of fetal bovine sera (FBS), calf, or horse sera.

应用

Minimum Essential Medium Eagle has been used:

• in the isolation and culture of primary hepatocytes
• to culture the human endometrial carcinoma cells Ishikawa (ATTC) and L-Wnt-3a cells (ATCC) that secrete Wnt family member 3a (Wnt-3a) ligand
• to culture the AN3CA (endometrial cancer cell line) cells, and HEC1-B human (endometrial adenocarcinoma cell line) cells

特点和优势

• Minimum Essential Medium Eagle (MEM) contains higher concentrations of amino acids to mimic the protein composition of cultured mammalian cells.
• The formulation has been altered by optional elimination of calcium to enable the growth of cells in suspension culture.

数量

按照每升培养基9.5克粉末配制。

重悬

补充2.2 g/L碳酸氢钠。

其他说明

除了以粉末形式提供外，基本上与原始配方相同。

Safety Information

• 储存分类代码: 13 - Non Combustible Solids
• WGK: WGK 2
• 闪点(F): Not applicable
• 闪点(C): Not applicable