Merck
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M0325

Sigma-Aldrich

Eagle最低必需培养基

With Earle′s salts, non-essential amino acids, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):
MEM
NACRES:
NA.75

质量水平

无菌性

sterile-filtered

形式

liquid

technique(s)

cell culture | mammalian: suitable

杂质

Endotoxin, tested

组分

glucose: 1.0 g/L (Dextro)
L-glutamine: 0.292 g/L
phenol red: 0.011 g/L
Earle’s salts (5% CO2): yes
NaHCO3: 2.2 g/L

运输

ambient

储存温度

2-8°C

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sterility

sterile-filtered

sterility

sterile-filtered

sterility

-

sterility

sterile-filtered

form

liquid

form

liquid

form

powder

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

Endotoxin, tested

impurities

endotoxin, tested

impurities

-

impurities

endotoxin, tested

components

glucose: 1.0 g/L (Dextro)
L-glutamine: 0.292 g/L
phenol red: 0.011 g/L
Earle’s salts (5% CO2): yes
NaHCO3: 2.2 g/L

components

L-glutamine: no
phenol red: yes
HEPES: 25 mM
sodium pyruvate: no
Earle’s salts (5% CO2): yes
NaHCO3: yes

components

NaHCO3: no
L-glutamine: yes
phenol red: yes
HEPES: no
sodium pyruvate: yes

components

glucose: yes
phenol red: yes
HEPES: no
Hanks’ salts (2% CO2): no
L-glutamine: no
NaHCO3: yes
Earle’s salts (5% CO2): no
sodium pyruvate: no
stable glutamine: no

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

一般描述

Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It is used for the cultivation of a wide variety of cells grown in monolayers. MEM includes higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. May be preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium.

应用

Minimum Essential Medium Eagle has been used as a culture medium for:
  • thyroid cancer cell lines
  • macrophage-like DH82 cells
  • vero CCL-81 cells

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

Certificate of Analysis

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Certificate of Origin

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