Merck

M0446

Sigma-Aldrich

StableCell MEM

With Earle′s salts, stable glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

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别名:
Eagle最低必需培养基, MEM
NACRES:
NA.75

质量水平

无菌性

sterile-filtered

形式

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

sodium pyruvate: no
HEPES: no
Hanks’ salts (2% CO2): no
NaHCO3: yes
Earle’s salts (5% CO2): no
stable glutamine: yes
phenol red: yes
L-glutamine: no
glucose: yes

运输

ambient

储存温度

2-8°C

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此商品
M4655FG032551416C
Sigma-Aldrich

M0446

StableCell MEM

Sigma-Aldrich

M4655

Eagle最低必需培养基

SAFC

FG0325

Minimum Essential Medium Eagle

SAFC

51416C

最低必需培养基

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

components

sodium pyruvate: no
HEPES: no
Hanks’ salts (2% CO2): no
NaHCO3: yes
Earle’s salts (5% CO2): no
stable glutamine: yes
phenol red: yes
L-glutamine: no
glucose: yes

components

sodium pyruvate: no
phenol red: yes
Earle’s salts (5% CO2): yes
HEPES: no
NaHCO3: yes
L-glutamine: yes

components

stable glutamine: yes
L-glutamine: no
phenol red: yes
sodium pyruvate: no
Earle’s salts (5% CO2): yes
HEPES: no
Hanks’ salts (2% CO2): no
glucose: yes
NaHCO3: yes

components

Earle’s salts (5% CO2): yes
L-glutamine: no

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

一般描述

由 Harry Eagle 开发的最低必需培养基(MEM)是所有合成细胞培养基中使用最广泛的培养基之一。
由 Harry Eagle 开发的最低必需培养基(MEM)是所有合成细胞培养基中使用最广泛的培养基之一。正常哺乳动物成纤维细胞和某些亚型的 HeLa 细胞的早期尝试培养表明,Eagle 基础培养基(BME)无法满足的他们的特殊营养需求。随后培养这些和其他细胞的研究表明,在 BME 中补充添加剂有利于更多种类的苛求细胞的生长。

MEM 包含了这些改进,包括更高浓度的氨基酸,因此培养基更接近于培养的哺乳动物细胞的蛋白质组成。MEM 已用于多种单层细胞的培养。在含有 Hanks 或 Earle 盐的配方中可选地补充非必需氨基酸,扩大了该培养基的用途。

应用

低限量 Eagle 培养基已用于小鼠成纤维细胞培养,用于体外细胞相容性测试。

其他说明

该产品不含 L-丙氨酸;L-天冬酰胺;L-谷氨酸;甘氨酸;L-脯氨酸;L-丝氨酸 和 L-谷氨酰胺。该产品补充了 L-Ala-L-Gln 二肽。这为细胞培养提供了更稳定的谷氨酰胺形式。游离氨基酸 L-谷氨酰胺在细胞培养中是不稳定的。

法律信息

StableCell is a trademark of Sigma-Aldrich Co. LLC

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves


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