Merck
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M3148

Sigma-Aldrich

2-巯基乙醇

for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

Synonym(s):
β-巯基乙醇, 2-羟乙基硫醇, BME, 硫代二甘醇
Linear Formula:
HSCH2CH2OH
CAS Number:
Molecular Weight:
78.13
Beilstein:
773648
EC 号:
MDL编号:
PubChem化学物质编号:
NACRES:
NA.31

等级

for molecular biology

质量水平

200

蒸汽密度

2.69 (vs air)

蒸汽压

1 mmHg ( 20 °C)

产品线

BioReagent

测定

99% (GC/titration)

形式

liquid

expl. lim.

18 %

浓度

14.3 M (pure liquid)

technique(s)

cell culture | mammalian: suitable
electrophoresis: suitable

折射率

n20/D 1.500 (lit.)

pH值(酸碱度)

4.5-6 (20 °C, 500 g/L)

bp

157 °C (lit.)

溶解性

H2O: soluble 1 mL/mL

密度

1.114 g/mL at 25 °C (lit.)

异质活性

DNase, RNase, protease, none detected

储存温度

room temp

SMILES string

OCCS

InChI

1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2

InChI key

DGVVWUTYPXICAM-UHFFFAOYSA-N

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Related Categories

应用

还原剂
β-巯基乙醇或2-巯基乙醇已用于:
  • 作为Roswell Park Memorial Institute (RPMI)-1640培养基的一种补充剂用于DT40细胞(鸡B细胞系)的培养
  • 裂解NuLi-1(Normal Lung, University of Iowa-1)细胞
  • 小鼠胚胎干细胞的胰蛋白酶消化
BME 适用于在聚丙烯酰胺凝胶电泳前还原蛋白质二硫键,通常用于 SDS-PAGE 的样品缓冲液,浓度为 5%。 切割分子间(亚基之间)二硫键允许蛋白质的亚基在 SDS-PAGE 上独立分离。 切割分子内(亚基内)二硫键可使亚基完全变性,从而使每个肽根据其链长迁移,而不受二级结构的影响。

包装

25, 100, 250, 500 mL in glass bottle

警示用语:

Danger

危险分类

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral

储存分类代码

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

闪点(F)

154.4 °F - closed cup

闪点(C)

68 °C - closed cup

个人防护装备

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Certificate of Analysis

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Certificate of Origin

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