Merck
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M3148

Sigma-Aldrich

2-巯基乙醇

for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

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别名:
β-巯基乙醇, 2-羟乙基硫醇, BME, 硫代二甘醇
线性分子式:
HSCH2CH2OH
CAS号:
60-24-2
分子量:
78.13
Beilstein:
773648
EC 号:
200-464-6
MDL编号:
MFCD00004890
PubChem化学物质编号:
24896800
NACRES:
NA.31

等级

for molecular biology

质量水平

200

蒸汽密度

2.69 (vs air)

蒸汽压

1 mmHg ( 20 °C)

产品线

BioReagent

检测方案

99% (GC/titration)

形式

liquid

expl. lim.

18 %

reaction suitability

reagent type: reductant

浓度

14.3 M (pure liquid)

technique(s)

cell culture | mammalian: suitable
electrophoresis: suitable

折射率

n20/D 1.500 (lit.)

pH值（酸碱度）

4.5-6 (20 °C, 500 g/L)

bp

157 °C (lit.)

溶解性

H2O: soluble 1 mL/mL

密度

1.114 g/mL at 25 °C (lit.)

异质活性

DNase, RNase, protease, none detected

储存温度

room temp

SMILES string

OCCS

InChI

1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2

InChI key

DGVVWUTYPXICAM-UHFFFAOYSA-N

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此商品
63689M625097622
2-Mercaptoethanol for molecular biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

Sigma-Aldrich

M3148

2-巯基乙醇

2-Mercaptoethanol BioUltra, for molecular biology, ≥99.0% (GC)

Sigma-Aldrich

63689

2-巯基乙醇

2-Mercaptoethanol ≥99.0%

Sigma-Aldrich

M6250

2-巯基乙醇

2-Mercaptoethanol for HPLC derivatization, LiChropur™, ≥99.0% (GC)

Supelco

97622

2-巯基乙醇

product line

BioReagent

product line

BioUltra

product line

-

product line

-

assay

99% (GC/titration)

assay

≥99.0% (GC)

assay

≥99.0%

assay

≥99.0% (GC)

form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable, electrophoresis: suitable

technique(s)

RNA extraction: suitable, protein extraction: suitable

technique(s)

-

technique(s)

HPLC: suitable

pH

4.5-6 (20 °C, 500 g/L)

pH

4.5-6 (20 °C, 500 g/L)

pH

4.5-6 (20 °C, 500 g/L)

pH

-

应用

还原剂
β-巯基乙醇或2-巯基乙醇已用于：
  • 作为Roswell Park Memorial Institute (RPMI)-1640培养基的一种补充剂用于DT40细胞（鸡B细胞系）的培养
  • 裂解NuLi-1（Normal Lung, University of Iowa-1）细胞
  • 小鼠胚胎干细胞的胰蛋白酶消化
BME 适用于在聚丙烯酰胺凝胶电泳前还原蛋白质二硫键，通常用于 SDS-PAGE 的样品缓冲液，浓度为 5%。 切割分子间（亚基之间）二硫键允许蛋白质的亚基在 SDS-PAGE 上独立分离。 切割分子内（亚基内）二硫键可使亚基完全变性，从而使每个肽根据其链长迁移，而不受二级结构的影响。

警示用语：

Danger

危险分类

Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral

储存分类代码

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

闪点(F)

154.4 °F - closed cup

闪点(C)

68 °C - closed cup

个人防护装备

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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A subset of platinum-containing chemotherapeutic agents kills cells by inducing ribosome biogenesis stress
Peter M Bruno
Nature Medicine, 23 (2017)
Reduced Innate Immune Response to a Staphylococcus aureus Small Colony Variant Compared to Its Wild-Type Parent Strain
Judy J
Frontiers in Cellular and Infection Microbiology, 6, 187-187 (2016)
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Yasunari Seita et al.
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Nonhuman primates (NHPs) are considered to be the most valuable models for human transgenic (Tg) research into disease because human pathology is more closely recapitulated in NHPs than rodents. Previous studies have reported the generation of Tg NHPs that ubiquitously
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