M3148

2-巯基乙醇

Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)

• 别名: β-巯基乙醇, 2-羟乙基硫醇, BME, 硫代二甘醇
• 线性分子式: HSCH₂CH₂OH
• CAS号: 60-24-2
• 分子量: 78.13
• Beilstein: 773648
• EC 号: 200-464-6
• MDL编号: MFCD00004890
• UNSPSC代码: 12161504
• PubChem化学物质编号: 24896800
• NACRES: NA.31

属性

• 等级: Molecular Biology
• 蒸汽密度: 2.69 (vs air)
• 蒸汽压: 1 mmHg ( 20 °C)
• 产品线: BioReagent
• 方案: 99% (GC/titration)
• 表单: liquid
• expl. lim.: 18 %
• 反应适用性: reagent type: reductant
• 浓度: 14.3 M (pure liquid)
• 技术: cell culture | mammalian: suitable; electrophoresis: suitable
• 折射率: n20/D 1.500 (lit.)
• pH值（酸碱度）: 4.5-6 (20 °C, 500 g/L)
• 沸点: 157 °C (lit.)
• 溶解性: H₂O: soluble 1 mL/mL
• 密度: 1.114 g/mL at 25 °C (lit.)
• 异质活性: DNase, RNase, protease, none detected
• 储存温度: 2-8°C
• SMILES字符串: OCCS
• InChI: 1S/C2H6OS/c3-1-2-4/h3-4H,1-2H2
• InChI key: DGVVWUTYPXICAM-UHFFFAOYSA-N

说明

应用

还原剂
β-巯基乙醇或2-巯基乙醇已用于：

• 作为Roswell Park Memorial Institute (RPMI)-1640培养基的一种补充剂用于DT40细胞（鸡B细胞系）的培养
• 裂解NuLi-1（Normal Lung, University of Iowa-1）细胞
• 小鼠胚胎干细胞的胰蛋白酶消化

BME is suitable for reducing protein disulfide bonds prior to polyacrylamide gel electrophoresis and is usually included in a sample buffer for SDS-PAGE at a concentration of 5%. Cleaving intermolecular (between subunits) disulfide bonds allows the subunits of a protein to separate independently on SDS-PAGE. Cleaving intramolecular (within subunit) disulfide bonds allows the subunits to become completely denatured so that each peptide migrates according to its chain length with no influence due to secondary structure.

安全信息

• 象形图: GHS06,GHS08,GHS05,GHS09
• 警示用语：: Danger
• 危险声明: H301 + H331,H310,H315,H317,H318,H361f,H373,H410
• 预防措施声明: P273 - P280 - P301 + P310 - P302 + P352 + P310 - P304 + P340 + P311 - P305 + P351 + P338
• 危险分类: Acute Tox. 2 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1A - STOT RE 2 Oral
• 靶器官: Liver,Heart
• 储存分类代码: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• 闪点(°F): 154.4 °F - closed cup
• 闪点(°C): 68 °C - closed cup
• 个人防护装备: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter