M3546

Sigma-Aldrich

超低范围分子量标记物(M.W. 1,060-26,600)

NACRES:
NA.25

用途

 vial sufficient for 400 applications on large gels (16 × 18 cm)
 vial sufficient for 800 mini-gels (10 × 10 cm)

质量水平

200
100

包装

vial of 200 μL

储存温度

−20°C

相关类别

应用

超低分子量标记物 (M.W.1,060-26,600) 已作为蛋白质标记物用于十二烷基硫酸钠聚丙烯酰胺凝胶电泳 (SDS-PAGE) 和免疫印迹。

生化/生理作用

超低量程标记物设计用于 Tris-Tricine SDS-PAGE 方法 当用亮蓝 G(非甲醇基)染色时。建议使用超低标记物固定戊二醛,顺序使两种最小的蛋白质固定在凝胶中。超低量程标记物以液体形式提供,上样前必须用提供的样品缓冲液进行稀释 (1:20)。

外形

0.1 MTris-HCl 溶液,pH 值约为 8.5,与 4 mM EDTA,3 mM 叠氮化钠和 40% 甘油

警示用语:

Danger

hazcat

Acute Tox. 4 Dermal - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Sens. 1

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

分析证书

原产地证书 (CofO)

Production of a recombinant antimicrobial peptide in transgenic plants using a modified VMA intein expression system.
Morassutti C, et al.
Febs Letters, 519, 141-146 (2002)
Endostatin enhances radioresponse in breast cancer cells via alteration of substance P levels.
Arslan Aydemir E, et al.
Oncology Letters, 2, 879-886 (2011)
Christelle Richard et al.
Applied and environmental microbiology, 70(1), 248-254 (2004-01-09)
Polyclonal antibodies have been generated by immunization of rabbits with a chemically synthesized C-terminal part of divercin V41 (DvnCt) conjugated to the carrier protein keyhole limpet hemocyanin (KLH). The sensitivity and reactivity of the DvnCt-KLH-generated antibodies were evaluated by enzyme-linked...
Thiago Machado Pasin et al.
Applied biochemistry and biotechnology, 191(3), 1111-1126 (2020-01-22)
The use of non-potable water (such as seawater) is an attractive alternative for water intensive processes such as biomass pretreatment and saccharification steps in the production of biochemicals and biofuels. Identification and application of halotolerant enzymes compatible with high-salt conditions...
Raquel Soares Oliveira et al.
Toxins, 12(6) (2020-06-19)
The biological activity of Rhinella icterica parotoid secretion (RIPS) and some of its chromatographic fractions (RI18, RI19, RI23, and RI24) was evaluated in the current study. Mass spectrometry of these fractions indicated the presence of sarmentogenin, argentinogenin, (5β,12β)-12,14-dihydroxy-11-oxobufa-3,20,22-trienolide, marinobufagin, bufogenin...
实验方案
Separation of Polyacrylic acid (PAA) 438 kDa; Polyacrylic acid (PAA) 235 kDa; Polyethylenimine (PEI) 266 kDa ; Poly(dimethyl diallyl ammonium chloride) (PIDADMACl) 204 kDa; p-Aminosalicylic acid (PAS) 7800 Da; p-Aminosalicylic acid (PAS) 257 kDa; Cationic dextran (11 kDa); Chitosan (13.4 kDa)
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