M5774

Sigma-Aldrich

Anti-Mouse Serum antibody produced in rabbit

whole antiserum

MDL number:
NACRES:
NA.46

biological source

rabbit

Quality Level

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

contains

15 mM sodium azide

species reactivity

mouse

application(s)

immunoelectrophoresis: suitable

conjugate

unconjugated

shipped in

dry ice

storage temp.

−20°C

General description

Mouse serum comprises hormones, antigens, electrolytes and antibodies. It serves blocking agent in immunoassays.

Specificity

Strong reactivity with normal mouse serum has been determined by immunoelectrophoresis (IEP). This antiserum has not been assayed for interspecies crossreactivity.

Application

Anti-Mouse Serum antibody produced in rabbit has been used in differential fluorescent labelling. It has also been used in differential staining of inner cell mass (ICM) and and trophectoderm (TE) cells. It has been also been used in the removal of zona pellucidae for the isolation of inner cell mass (ICM) from mouse blastocysts.

Physical form

Rabbit Anti-Mouse Serum is provided as a liquid containing 15 mM sodium azide as preservative.

Storage and Stability

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution may be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Effects of paternal heat stress on the in vivo development of preimplantation embryos in the mouse
Zhu BK and Setchell BP
Reproduction, Nutrition, Development, 44(6), 617-629 (2004)
J Van der Elst et al.
Human reproduction (Oxford, England), 13(6), 1595-1599 (1998-08-04)
We demonstrated previously that ultra-rapid freezing of mouse oocytes with 3.5 M dimethylsulphoxide (DMSO) decreased cell numbers in day 5 in-vitro cultured blastocysts. In the present study we counted cell numbers of trophectoderm (TE) and inner cell mass (ICM) separately...
Namfon Inna et al.
Clinical and experimental reproductive medicine, 45(3), 110-115 (2018-09-12)
To compare our in-house method of embryo freezing with Cryotop vitrification in terms of immediate survival, subsequent cleavage and blastocyst formation, and cell numbers in blastocysts. Two-cell mouse embryos were randomly allocated into three groups: a non-frozen control group (group...
Usanee Sanmee et al.
Clinical and experimental reproductive medicine, 43(3), 152-156 (2016-10-01)
We studied the effect of the site of laser zona opening on the complete hatching of mouse blastocysts and the cell numbers of the completely hatched blastocysts. Mouse blastocysts were randomly allocated to the inner cell mass (ICM) group (zona...
Suo-Feng Ma et al.
Theriogenology, 64(5), 1142-1157 (2005-08-30)
Strontium has been successfully used to induce activation of mouse oocytes in nuclear transfer and other experiments, but the optimum treatment conditions have not been studied systematically. When cumulus-free oocytes were treated with 10mM SrCl(2) for 0.5-5h, activation rates (88.4+/-4.1...

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