Merck
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M6395

Sigma-Aldrich

MCDB 105 培养基

With trace elements, L-glutamine and 25mM HEPES, powder, suitable for cell culture

Synonym(s):
Molecular, Cellular, and Development Biology 105 medium
NACRES:
NA.75

质量水平

形式

powder

technique(s)

cell culture | mammalian: suitable

组分

phenol red: 0.001242 g/L
glucose: 0.72064 g/L (Dextro)
sodium pyruvate: 0.11 g/L
HEPES: 5.958 g/L (25mM)
L-glutamine: 0.3653 g/L

运输

ambient

储存温度

2-8°C

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MCDB 105 培养基

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form

powder

form

powder

form

powder

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

phenol red: 0.001242 g/L
glucose: 0.72064 g/L (Dextro)
sodium pyruvate: 0.11 g/L
HEPES: 5.958 g/L (25mM)
L-glutamine: 0.3653 g/L

components

NaHCO3: no
L-glutamine: 0.8772 g/L
glucose: 1.081 g/L (Dextro)
sodium pyruvate: 0.055 g/L
phenol red: 0.001242 g/L
HEPES: 6.6 g/L (25mM)

components

HEPES: 7.149 g/L (30mM)
glucose: 1.441 g/L (Dextro)
phenol red: 0.001242 g/L
sodium pyruvate: 0.055 g/L
L-glutamine: 0.14615 g/L

components

L-glutamine: no

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

500

Quality Level

500

Quality Level

500

Quality Level

400

一般描述

MCDB培养基专门用于特定细胞的低蛋白或无血清培养,需补充激素、生长因子、微量元素或低浓度的透析胎牛血清蛋白(FBSP)。每种MCDB培养基都经过特别的配方设计(定性和定量),提供成分确定和恰当平衡的营养环境,选择性促进特定细胞的生长。MCDB 105培养基是MCDB 104培养基的改良版。适合用于WI-38、MRC-5等人二倍体成纤维样细胞的长期培养。

应用

MCDB 105培养基已用于培养:
  • 人脐静脉内皮细胞
  • 原代人子宫内膜基底细胞(ESC)
  • 卵巢癌细胞株(OVCAR3)

数量

配制成每升培养基含 14.9 克粉末。

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable


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