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Merck

M7145

Sigma-Aldrich

MEM 非必需氨基酸溶液 (100×)

Without ʟ-glutamine, sterile liquid, suitable for cell culture

别名:

MEM NEAA

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About This Item

分類程式碼代碼:
12352207
eCl@ss:
32160406
NACRES:
NA.75

product name

MEM 非必需氨基酸溶液 (100×), without L-glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture

品質等級

無菌

sterile-filtered

產品線

BioReagent

形狀

liquid

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

儲存溫度

2-8°C

一般說明

Eagle′s基本必需培养基(MEM)是一种合成培养基,由平衡盐溶液、氨基酸和维生素组成。这些组份为培养细胞生长的必需组份。MEM可添加非必需氨基酸,而制备MEM非必需氨基酸溶液。非必需氨基酸包括L-丙氨酸、L-天冬酰胺、L-天冬氨酸、L-甘氨酸、L-丝氨酸、L-脯氨酸和L-谷氨酸。

應用

向培养基中添加氨基酸补充剂不仅可以刺激细胞生长还可以延长培养细胞的活力。MEM非必需氨基酸溶液的补充物,可增加营养成分并减少 体外细胞的生物合成负担。
MEM非必需氨基酸溶液已可用于维持哺乳动物细胞的Dulbecco′s基本必需培养基。它已被添加到卵母细胞成熟培养基中。

生化/生理作用

杂交瘤培养研究表明,Eagle8242;s基本培养基(MEM)添加非必需氨基酸可提高杂交瘤细胞在死亡阶段的膜稳定性。这也会导致死亡阶段抗体分泌增加,乳酸浓度下降。

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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