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M7403

Sigma-Aldrich

MCDB 153培养基

With trace elements, L-glutamine and 28mM HEPES, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):
Molecular, Cellular, and Development Biology 153 medium
NACRES:
NA.75

质量水平

形式

powder

technique(s)

cell culture | mammalian: suitable

组分

NaHCO3: no
L-glutamine: 0.8772 g/L
glucose: 1.081 g/L (Dextro)
sodium pyruvate: 0.055 g/L
phenol red: 0.001242 g/L
HEPES: 6.6 g/L (25mM)

运输

ambient

储存温度

2-8°C

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MCDB 153 Medium

form

powder

form

powder

form

powder

form

liquid

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

NaHCO3: no
L-glutamine: 0.8772 g/L
glucose: 1.081 g/L (Dextro)
sodium pyruvate: 0.055 g/L
phenol red: 0.001242 g/L
HEPES: 6.6 g/L (25mM)

components

phenol red: 0.001242 g/L
glucose: 0.72064 g/L (Dextro)
sodium pyruvate: 0.11 g/L
HEPES: 5.958 g/L (25mM)
L-glutamine: 0.3653 g/L

components

HEPES: 7.149 g/L (30mM)
glucose: 1.441 g/L (Dextro)
phenol red: 0.001242 g/L
sodium pyruvate: 0.055 g/L
L-glutamine: 0.14615 g/L

components

L-glutamine: no

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

500

Quality Level

500

Quality Level

500

Quality Level

400

一般描述

MCDB 153培养基是对Ham营养混合物F-12的改良,设计用于人角质形成细胞的生长、鸡胚成纤维细胞的克隆生长和中国仓鼠卵巢(CHO)细胞的生长,使用低水平的胎牛血清蛋白(FBSP)、大量微量元素或无血清蛋白。

应用

MCDB 153培养基已用作黑色素瘤分离培养基(MIM)的组分,用于培养人类非转移性和转移性黑色素瘤细胞。

数量

配方中每升培养基含有17.6克的粉末。

重悬

并补充1.18 g/L碳酸氢钠。

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable


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