Merck
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M8403

Sigma-Aldrich

McCoy 5A培养基

Modified, with sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture

别名:
5A basal medium
EC 号:
MDL编号:
NACRES:
NA.75

质量水平

400

无菌性

sterile-filtered

形式

liquid

technique(s)

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

NaHCO3: 2.2 g/L
phenol red: 0.011 g/L
L-glutamine: no
glucose: 3.0 g/L
glucose: 3 g/L

运输

ambient

储存温度

2-8°C

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应用

用于来自各种组织的细胞的原代培养。推荐用于在原代细胞培养物中生产病毒。

生化/生理作用

起初是通过修改BME中的氨基酸,用来支持肝肿瘤细胞。 该配方还被用来培养骨髓,皮肤,牙龈,肾脏,大网膜,肾上腺,肺,脾,大鼠胚胎和其他细胞类型。

重悬

补充 0.22 g/L L-谷氨酰胺。

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

分析证书

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原产地证书 (CofO)

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Seungman Park et al.
Scientific reports, 9(1), 13286-13286 (2019-09-19)
We present a high-throughput microfluidics technique facilitating in situ measurements of cell mechanics parameters at the dorsal side of the cell, including molecular binding strengths, local traction forces, and viscoelastic properties. By adjusting the flow rate, the force magnitude exerted
Katsuhiro Uzawa et al.
Scientific reports, 4, 5433-5433 (2014-06-26)
No definitive therapy exists to treat human metastatic tumors. We reported previously that down-regulation of Lin-7C is essential for metastasis of human squamous cell carcinomas (hSCCs). In this study, we investigated the chemical restoration of Lin-7C expression and demonstrated its
Claudia De Fusco et al.
Journal of medicinal chemistry, 64(10), 6814-6826 (2021-04-27)
MAT2a is a methionine adenosyltransferase that synthesizes the essential metabolite S-adenosylmethionine (SAM) from methionine and ATP. Tumors bearing the co-deletion of p16 and MTAP genes have been shown to be sensitive to MAT2a inhibition, making it an attractive target for
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Huw D Summers et al.
ACS nano, 7(7), 6129-6137 (2013-06-19)
Assessing dose in nanoparticle-cell interactions is inherently difficult due to a complex multiplicity of possible mechanisms and metrics controlling particle uptake. The fundamental unit of nanoparticle dose is the number of particles internalized per cell; we show that this can

技术文章

McCoy's 5A Modified Media Formulation

McCoy's 5A Modified Media Formulation

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