Merck
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M8537

Sigma-Aldrich

MCDB 131培养基

With trace elements and L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):
Molecular, Cellular, and Development Biology 131 medium
NACRES:
NA.75

形式

powder

浓度

11.6 g/L

technique(s)

cell culture | mammalian: suitable

组分

sodium pyruvate: 0.11 g/L
phenol red: 0.012 g/L
L-glutamine: 1.461 g/L
HEPES: no
glucose: 1.0 g/L (Dextro)

运输

ambient

储存温度

2-8°C

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form

powder

form

powder

form

powder

form

powder

concentration

11.6 g/L

concentration

-

concentration

-

concentration

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: 0.11 g/L
phenol red: 0.012 g/L
L-glutamine: 1.461 g/L
HEPES: no
glucose: 1.0 g/L (Dextro)

components

NaHCO3: no
L-glutamine: 0.8772 g/L
glucose: 1.081 g/L (Dextro)
sodium pyruvate: 0.055 g/L
phenol red: 0.001242 g/L
HEPES: 6.6 g/L (25mM)

components

phenol red: 0.001242 g/L
glucose: 0.72064 g/L (Dextro)
sodium pyruvate: 0.11 g/L
HEPES: 5.958 g/L (25mM)
L-glutamine: 0.3653 g/L

components

HEPES: 7.149 g/L (30mM)
glucose: 1.441 g/L (Dextro)
phenol red: 0.001242 g/L
sodium pyruvate: 0.055 g/L
L-glutamine: 0.14615 g/L

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

一般描述

MCDB-131培养基提供了一个清晰和平衡的营养环境,可选择性地促进特定细胞类型的生长。MCDB-131在补充0.7% FBSP、10 ng/ml表皮生长因子、1 μg/ml氢化可的松后,可支持人微血管内皮细胞(包括人脐静脉内皮细胞)的克隆性生长。MCDB培养基用于特定类型细胞的低蛋白或无血清生长,使用的是激素、生长因子、微量元素或低水平透析胎牛血清蛋白(FBSP)。 

应用

MCDB-131培养基已用于:

  • 作为牛视网膜内皮细胞基础培养基的组分
  • 培养牛肺微血管内皮细胞
  • 分离猪颅内内皮细胞和猪主动脉内皮细胞

数量

按照每升培养基11.6克粉末配制。

重悬

补充1.18 g/L碳酸氢钠。

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable


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