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M9274

Sigma-Aldrich

Murashige & Skoog 基本培养基

suitable for plant cell culture, with sucrose and agar

别名:
MS 基本培养基
MDL编号:
NACRES:
NA.72

质量水平

200

形式

powder

应用

cell culture | plant: suitable

特色行业

Agriculture

运输

ambient

储存温度

2-8°C

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应用

Murashige & Skoog 基本培养基已用于培养拟南芥转基因种子以促进子叶发育,培养珍珠粟 (Pennisetum glaucum) 和迷迭香( Rosmarinus ofcinalis L.) 以诱导愈伤组织。

公式变量

如Murashige和Skoog (1962)描述的含有大量和微量营养素、维生素、蔗糖和琼脂。

培养基配方

数量

配制成每升培养基含 42.4 克粉末。

象形图

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警示用语:

Warning

危险声明

Hazard Classifications

Eye Irrit. 2 - Ox. Sol. 3

Storage Class Code

5.1B - Oxidizing hazardous materials

WGK

WGK 2

闪点(F)

Not applicable

闪点(C)

Not applicable

分析证书

原产地证书 (CofO)

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the stability of the prepared Murashige and Skoog Basal Medium, Product M9274?

    This agar-containing medium can be stored for 3 to 6 months, depending on the stability of any additional supplements, if stored properly with no observable dehydration of the gel. If the medium appears to darken or pull away from the sides of the culture vessel, it should be discarded.

  6. How is Murashige and Skoog Basal Medium, Product M9274 different from Product M5519?

    M9274 contains sucrose at 30 g/L and agar at 8 g/L; M5519 does not.

  7. How firm is the gel in the final sterile medium preparation of Murashige and Skoog Basal Medium, Product M9274?

    This formulation contains 8g/L of standard agar resulting in fairly firm and rigid culture medium.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Antoine Fort et al.
The New phytologist, 209(2), 590-599 (2015-09-24)
Heterosis is the phenomenon whereby hybrid offspring of genetically divergent parents display superior characteristics compared with their parents. Although hybridity and polyploidy can influence heterosis in hybrid plants, the differential contributions of hybridity vs polyploidy to heterosis effects remain unknown....
Striking effects of melatonin on secondary metabolites produced by callus culture of rosemary (Rosmarinus officinalis L.)
Coskun Y, et al.
Plant Cell, Tissue and Organ Culture, 138(1), 89-95 (2019)
Baba Ngom et al.
Plant signaling & behavior, 12(9), e1356967-e1356967 (2017-08-02)
Cytosine DNA methylation is an epigenetic regulatory system used by plants to control gene expression. Methylation pattern always changes after abiotic stresses, pathogens and pest infections or after a treatment with salicylic acid (SA). The latter is a key player...
Laure Audonnet et al.
Gene expression patterns : GEP, 25-26, 1-7 (2017-04-13)
Dimethylation of histone H3 lysine 9 (H3K9me2) is a heterochromatic mark linked to DNA methylation and gene repression. Removal of H3K9me2 from gene bodies by the jmjC histone demethylase IBM1/JMJ25 inhibits DNA methylation and derepresses gene expression. In this work...
Smita P Chavan et al.
Applied microbiology and biotechnology, 89(6), 1701-1707 (2010-12-02)
The present study examined the effects of plant growth hormones, incubation period, biotic (Trametes versicolor, Mucor sp., Penicillium notatum, Rhizopus stolonifer, and Fusarium oxysporum) and abiotic (NaCl, MgSO(4), FeSO(4), ZnSO(4), and FeCl(3)) elicitors on cell growth and α-tocopherol and pigment...

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