Merck
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M9309

Sigma-Aldrich

McCoy 5A培养基

Modified, with L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

Synonym(s):
5A basal medium
MDL编号:

质量水平

400

无菌性

sterile-filtered

形式

liquid

technique(s)

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

L-glutamine: 0.21 g/L
phenol red: 0.011 g/L
NaHCO3: 2.2 g/L
glucose: 3.0 g/L (Dextro)

运输

ambient

储存温度

2-8°C

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一般描述

The amino acid requirements for in vitro cultivation of Novikoff hepatoma cells were reported by McCoy and colleagues. These experiments were carried out using Basal Medium 5A, which was later modified to create McCoy′s 5A medium.

应用

McCoy′s 5A Medium has been used:
  • to culture HCT-116?cells
  • to culture clear cell renal cell carcinoma (ccRCC) cells Caki-1
  • to maintain and culture human bone osteosarcoma Saos-2 cell line for in vitro cell studies

生化/生理作用

起初是通过修改BME中的氨基酸,用来支持肝肿瘤细胞。 该配方还被用来培养骨髓,皮肤,牙龈,肾脏,大网膜,肾上腺,肺,脾,大鼠胚胎和其他细胞类型。

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

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