Merck

MAK116

Sigma-Aldrich

肌酸激酶活性检测试剂盒

sufficient for 100 tests (Ultraviolet)

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别名:
肌酸激酶活性检测试剂盒, 肌酸磷酸激酶活性检测试剂盒
NACRES:
NA.84

用途

sufficient for 100 tests (Ultraviolet)

检测方法

colorimetric

相关疾病

cardiovascular diseases; genitourinary disorders; aging/geriatric diseases; orthopedic diseases

运输

dry ice

储存温度

−20°C

基因信息

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MAK078

黄嘌呤氧化酶活性检测试剂盒

usage

sufficient for 100 tests (Ultraviolet)

usage

sufficient for 500 colorimetric tests

usage

sufficient for 100 colorimetric or fluorometric tests

usage

sufficient for 100 colorimetric or fluorometric tests

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric, fluorometric

detection method

colorimetric, fluorometric

shipped in

dry ice

shipped in

-

shipped in

dry ice

shipped in

-

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

relevant disease(s)

cardiovascular diseases; genitourinary disorders; aging/geriatric diseases; orthopedic diseases

relevant disease(s)

hematological disorder; cancer

relevant disease(s)

-

relevant disease(s)

rheumatological diseases; gastrointestinal diseases; cardiovascular diseases

一般描述

肌酸激酶(CK),又称磷酸肌酸激酶,是催化一个磷酸基团从ATP转移到肌酸,产生磷酸肌酸的酶,磷酸肌酸是肌肉和脑组织的重要能量储备。CK是一种由B(大脑)和M(肌肉)亚基组成的二聚体蛋白。现已发现三种同工酶,即:CK-MM、CK-MB和CK-BB。在心肌梗塞、横纹肌溶解、肌营养不良和肾功能衰竭等各种病理状况下,CK水平升高。

特点和优势

兼容高通量的操作系统。

适用性

适用于检测多种样品中的肌酸激酶活性,如血液、血清和血浆等。该试剂盒可用于研究多种化合物对肌酸激酶活性的影响。

原理

通过偶联酶反应确定肌酸激酶活性,在340 nm处测量反应产生的NADPH,结果与样品中的CK活性成比例。在此反应中,磷酸肌酸和ADP转化为肌酸和ATP。己糖激酶将产生的ATP用于磷酸化葡萄糖,生成葡萄糖-6-磷酸;在葡萄糖-6-磷酸脱氢酶存在的情况下,NADP氧化葡萄糖-6-磷酸,生成NADPH和6-磷酸-D-葡萄糖酸。一个单位的CK指pH 6.0时,每分钟将1.0μmole磷酸盐从磷酸肌酸转移到ADP的酶量。 该试剂盒的线性范围为30-1,800 单位/L CK活性。

相关产品

产品编号
说明
价格

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 3


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