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MAK404

Sigma-Aldrich

Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric)

sufficient for 100 colorimetric tests

质量水平

detection method

colorimetric

储存温度

−20°C

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Carbonic Anhydrase (CA) Activity Assay Kit (Colorimetric)|Sigma-Aldrich

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Phosphoenolpyruvate Carboxykinase Activity Assay Kit (Colorimetric)|Sigma-Aldrich

-

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

detection method

colorimetric

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

100

Quality Level

-

Quality Level

100

Quality Level

-

一般描述

Carbonic anhydrases (CA) are zinc enzymes present in both prokaryotes and eukaryotes. They catalyze efficiently the reversible hydration of CO2 to bicarbonate. Their important patho-physiological roles in respiration, pH and CO2 homeostasis, secretion, gluconeogenesis, ureagenesis etc. make it an important drug target. Characteristic increase and decrease of CA Activity is observed in different physiological conditions and diseases such as anemia, Thalassemia, Hypothyroidism, Hyperthyroidism, and in several cases of lung and liver diseases and Leukemia.

特点和优势

Carbonic Anhydrase (CA) Activity Assay Kit:
  • Compatible with high-throughput handling systems.

适用性

The kit is suitable for the detection of carbonic anhydrase activity in hemolysates and serum, as well as the determination of enzymatic activity of purified CA.

原理

The assay utilizes the esterase activity of an active CA on an ester substrate which releases a chromophore.The released product can be easily quantified using a spectrophotometric multi-well plate reader. In the presence of a CA specific inhibitor, the enzyme loses its activity which results in a decrease of absorbance.To measure specific CA activity, the CA inhibitor Acetazolamide is included.Acetazolamide is a potent inhibitor of CA I, CA II, CA IV, CA IX, CA XII etc. except CA III.

象形图

CorrosionHealth hazard

警示用语:

Danger

危险声明

危险分类

Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2

储存分类代码

10 - Combustible liquids

WGK

WGK 3


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