MAK439

Glycolysis Assay Kit

Sufficient for 100 colorimetric tests

Properties

• 质量水平: 100
• detection method: colorimetric
• 相关疾病: diabetes; cancer; endocrinological disorders
• 储存温度: −20°C

Description

一般描述

Glycolysis is one of the major metabolic pathways cells undergo to produce energy and results in the production of pyruvate. One of the eventual end products of this process is lactate dehydrogenase-mediated conversion of pyruvate to L-lactate via lactic acid fermentation. This enzymatic mechanism allows L-lactate to serve as an indicator of glycolysis.

特点和优势

• Linear detection range up to 10 mM L-lactate in 96-well plate assay.
• Convenient. The procedure is performed at room temperature and involves adding a single working reagent.
• Compatible with high-throughput handling systems.

适用性

The kit is suitable for the direct determination of L-Lactate produced by glycolysis in cell media samples and for the screening of glycolysis inhibitors.

原理

The Glycolysis Assay Kit is based on measuring the production of L-Lactate from glycolysis in cells. L-Lactate that is secreted into the cell media is quantified using a coupled reaction involving the lactate dehydrogenase catalyzed oxidation of L-lactate that generates pyruvate and NADH, which subsequently reduces a formazan dye. The intensity of the reduced dye, measured at 565 nm, is directly proportional to the L-lactate concentration in the sample, which in turn is directly proportional to the glycolytic rate of the cells.

Safety Information

• 储存分类代码: 12 - Non Combustible Liquids
• WGK: WGK 2