Merck
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MITOISO1

Sigma-Aldrich

线粒体分离试剂盒

sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues

EC 号:
NACRES:
NA.32

质量水平

200

用途

sufficient for 10-20 g (animal tissue)
sufficient for 50 assays (2 mL)

保质期

2 yr

运输

dry ice

储存温度

−20°C

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应用

The Mitochondria Isolation Kit provides a fast and easy isolation of an enriched mitochondrial fraction from animal tissues as well as the testing of the electrochemical proton gradient (ΔΨ) of the inner mitochondrial membrane. Useful for mitochondria mediated apoptosis studies.
Mitochondria Isolation Kit has been used:
  • for the separation of mitochondrial and cytosolic fraction for determining the branched-chain aminotransferase (BCAT) activity
  • for the isolation of mitochondria from glomeruli and heart
  • for the isolation of mitochondria from injured brain samples for the measurement of mitochondrial transmembrane potential

特点和优势

  • 特别配置的提取试剂和成熟的实验方案,适于小规模的分离 - 通过微量离心管分离富集的完整线粒体组分。
  • 可制备功能活性、完整的线粒体 - 所制得的线粒体适于体外检测,可用于凋亡、氧化应激及其他研究。
  • 包含可测定线粒体内膜完整性的染色剂和实验方案 - 无需购买其他试剂,即可测定线粒体内膜的完整性。
  • 兼容细胞色素c氧化酶检测试剂盒 - 可以轻松测定线粒体外膜完整性

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • T9201Trypsin from bovine pancreas, powder, ≥7,500 BAEE units/mg solid

象形图

Exclamation markHealth hazard

警示用语:

Danger

危险分类

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

10 - Combustible liquids

WGK

WGK 3

分析证书

请输入批号搜索分析证书(COA)。

原产地证书 (CofO)

请输入批号搜索原产地证书(COO)。

Product Information Sheet

Quotes and Ordering

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. Can Product MITOISO1, Mitochondria Isolation Kit, be used for isolation of mitochondria from cultured cells?

    It is possible to use this kit for cultured cells, however MITOISO2 is a kit that was specifically designed for this purpose.  This kit was originally designed for isolation from tissues.

  3. Are the extraction buffers from Product MITOISO1, Mitochondria Isolation Kit, available separately?

    The extraction buffers supplied with this kit are only available with the kit.

  4. When using Product MITOISO1, Mitochondria Isolation Kit, how long can I store my isolated mitochondria?

    If only interested in the proteins isolated from mitochondria, storage at  -20 deg C is recommended.  If intact mitochondria are required, storage at 4 deg C is recommended.  These intact mitochondria are stable after refrigeration for up to 24 hours.

  5. How much tissue can I use with Product MITOISO1, Mitochondria Isolation Kit,?

    The procedures described in the bulletin are for small amounts of tissue samples (50-200 mg). Larger amounts of tissue can be purified with modifications listed in the technical bulletin.  We recommend a minimum of 50 mg.

  6. Is the Product MITOISO1, Mitochondria Isolation Kit,  JC-1 stain available separately?

    Yes, while the component J4519 is not available separately, JC-1 stain is listed separately as Sigma Product No. T4069 or Product No. 40882.

  7. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  8. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  9. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  10. When using Product MITOISO2, Mitochondria Isolation Kit, how can I store my isolated mitochondria?

    Please see below for information on how best to store your isolated mitochondria using the MITOISO2 kit:If only interested in the proteins isolated from mitochondria, storage at  -20 deg C is recommended.  If intact mitochondria are required, storage at 4 deg C is recommended.  These intact mitochondria are stable after refrigeration for up to 24 hours.

  11. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Mitochondrial dysfunction mediates aldosterone-induced podocyte damage: a therapeutic target of PPARgamma
Zhu C, et al.
The American Journal of Pathology, 178(5), 2020-2031 (2011)
Young-Kyoung Shin et al.
Cancer research, 65(8), 3162-3170 (2005-04-19)
5-Fluorouracil (5-FU) is widely used for treatment of advanced colorectal cancer. However, it is common for such patients to develop resistance to 5-FU, and this drug resistance becomes a critical problem for chemotherapy. The mechanisms underlying this resistance are largely
DJ-1 protects the heart against ischemia-reperfusion injury by regulating mitochondrial fission
Shimizu Y, et al.
Journal of Molecular and Cellular Cardiology, 97, 56-66 (2016)
Emilyn U Alejandro et al.
Endocrinology, 151(2), 502-512 (2010-01-09)
Insulin enhances the proliferation and survival of pancreatic beta-cells, but its mechanisms remain unclear. We hypothesized that Raf-1, a kinase upstream of both ERK and Bad, might be a critical target of insulin in beta-cells. To test this hypothesis, we
Neuroprotective effect of hyperbaric oxygen therapy in brain injury is mediated by preservation of mitochondrial membrane properties
Palzur E, et al.
Brain Research, 1221, 126-133 (2008)

技术文章

Organelle Isolation

The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify organelle fractions from gently homogenized samples.

Apoptosis Assays

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Mitochondrial Stress and ROS

Oxidative stress is mediated, in part, by reactive oxygen species produced by multiple cellular processes and controlled by cellular antioxidant mechanisms such as enzymatic scavengers or antioxidant modulators. Free radicals, such as reactive oxygen species, cause cellular damage via cellular.

相关内容

线粒体应激和ROS

氧化应激,在一部分上,是由多种细胞过程产生的活性氧介导,并由诸如酶清除剂或抗氧化剂调节剂等细胞抗氧化剂机制所控制的。自由基,如活性氧,会通过细胞引起细胞损伤。

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