Merck

MITOISO1

Sigma-Aldrich

线粒体分离试剂盒

sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues

EC 号:
NACRES:
NA.32

质量水平

用途

sufficient for 10-20 g (animal tissue)
sufficient for 50 assays (2 mL)

保质期

2 yr

运输

dry ice

储存温度

−20°C

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此商品
LYSISO1MITOISO2CS0390
Mitochondria Isolation Kit sufficient for 10-20 g (animal tissue), sufficient for 50 assays (2 mL), isolation of enriched mitochondrial fraction from animal tissues

Sigma-Aldrich

MITOISO1

线粒体分离试剂盒

Sigma-Aldrich

Sigma-Aldrich

LYSISO1

溶酶体分离试剂盒

Mitochondria Isolation Kit sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells

Sigma-Aldrich

MITOISO2

线粒体分离试剂盒

Mitochondria Staining Kit 1 kit sufficient for 40 tests (of 5 mL cell suspensions), 1 kit sufficient for 200 tests (of 1 mL cell suspensions)

Sigma-Aldrich

CS0390

线粒体染色试剂盒

shelf life

2 yr

shelf life

-

shelf life

-

shelf life

-

shipped in

dry ice

shipped in

-

shipped in

dry ice

shipped in

wet ice

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

应用

线粒体分离试剂盒可简单、快速地分离动物组织中富集的线粒体组分,并检测线粒体内膜的电化学质子梯度(ΔΨ)。用于线粒体介导的细胞凋亡研究。
线粒体分离试剂盒已用于:
  • 分离线粒体和胞浆组分,用于测定支链氨基酸转氨酶(BCAT)活性
  • 分离肾小球和心脏线粒体
  • 分离脑损伤样品中的线粒体,检测线粒体跨膜电位

特点和优势

  • 特制提取剂和可靠实验方案适合少量分离——在离心管中即可分离富集完整线粒体组分。
  • 获得完整有活性的线粒体——所得线粒体适用于细胞凋亡、氧化应激等体外分析研究。
  • 提供可确定内膜完整性的染色剂和实验方案——无需购买其他试剂,即可确定线粒体内膜完整性。
  • 适配细胞色素C氧化酶检测试剂盒——可轻松确定线粒体外膜完整性。

试剂盒组分也可单独购买

产品编号
说明
化学品安全说明书

  • T9201Trypsin from bovine pancreas, powder, ≥7,500 BAEE units/mg solid化学品安全说明书

象形图

Exclamation markHealth hazard

警示用语:

Danger

危险分类

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

10 - Combustible liquids

WGK

WGK 3


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细胞器分离

亚细胞组分离心分离是一种常用技术,广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同,因此通过离心,可从温和均质的样品中轻松分离和纯化细胞器组分。

Organelle Isolation

The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify organelle fractions from gently homogenized samples.

凋亡分析检测

细胞凋亡测定使用膜联蛋白V、Caspase和TUNEL DNA片段化检测法检测程序性细胞死亡。

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Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

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