Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
10 - Combustible liquids
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
It is possible to use this kit for cultured cells, however MITOISO2 is a kit that was specifically designed for this purpose. This kit was originally designed for isolation from tissues.
The extraction buffers supplied with this kit are only available with the kit.
If only interested in the proteins isolated from mitochondria, storage at -20 deg C is recommended. If intact mitochondria are required, storage at 4 deg C is recommended. These intact mitochondria are stable after refrigeration for up to 24 hours.
The procedures described in the bulletin are for small amounts of tissue samples (50-200 mg). Larger amounts of tissue can be purified with modifications listed in the technical bulletin. We recommend a minimum of 50 mg.
Yes, while the component J4519 is not available separately, JC-1 stain is listed separately as Sigma Product No. T4069 or Product No. 40882.
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Please see below for information on how best to store your isolated mitochondria using the MITOISO2 kit:If only interested in the proteins isolated from mitochondria, storage at -20 deg C is recommended. If intact mitochondria are required, storage at 4 deg C is recommended. These intact mitochondria are stable after refrigeration for up to 24 hours.
Ask a Scientist here.
The isolation of subcellular fractions by centrifugation is a commonly used technique and is widely applicable across multiple cell and tissue types. Because organelles differ in their size, shape, and density, centrifugation can be easily employed to separate and purify organelle fractions from gently homogenized samples.
Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
Oxidative stress is mediated, in part, by reactive oxygen species produced by multiple cellular processes and controlled by cellular antioxidant mechanisms such as enzymatic scavengers or antioxidant modulators. Free radicals, such as reactive oxygen species, cause cellular damage via cellular.