- MLMIR0720
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质量水平
产品线
MISSION®
形式
liquid
浓度
≥1x106 VP/ml (via p24 assay)
成熟序列
AGAGGCUUAUAGCUCUAAGCC
Sanger mature/minor 登记号
Sanger microRNA登记号
运输
dry ice
储存温度
−70°C
Related Categories
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This Item | MLMIR0049 | MLMIR0067 | MLMIR0035 |
|---|---|---|---|
| product line MISSION® | product line MISSION® | product line MISSION® | product line MISSION® |
| concentration ≥1x106 VP/ml (via p24 assay) | concentration ≥1x106 VP/ml (via p24 assay) | concentration ≥1x106 VP/ml (via p24 assay) | concentration ≥1x106 VP/ml (via p24 assay) |
| mature sequence AGAGGCUUAUAGCUCUAAGCC | mature sequence ACUCCAUUUGUUUUGAUGAUGG | mature sequence GAGGGAGGGACGGGGGCGGUGC | mature sequence CGGGGCCGUAGCACUGUCUGA |
| Sanger mature/minor accession no. | Sanger mature/minor accession no. | Sanger mature/minor accession no. | Sanger mature/minor accession no. |
| Sanger microRNA accession no. | Sanger microRNA accession no. | Sanger microRNA accession no. | Sanger microRNA accession no. |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
一般描述
Sigma 公司的 Mission Lenti-miRs 表达miRNA 来自一副共同的主干，其结构满足精确切割处理的要求，部分互补链旨在使用专有算法模拟主干结构中的碱基配对模式。将含有 microRNA 序列的寡核苷酸克隆到 TRC2-pLKO-puro 载体中。每个 miRNA 构建体都已被克隆和序列验证。 成熟的 microRNA 序列从 miRBase 获得。慢病毒转导颗粒由序列验证的慢病毒质粒载体产生。将含有 microRNA 序列的寡核苷酸克隆到 TRC2-pLKO-puro 载体中。将该载体与兼容的包装质粒共转染到适当的细胞系中，产生可用于转导哺乳动物细胞的病毒颗粒。聚合酶 II 启动子、延伸因子 1 α（EF1A）被选择用来驱动病毒 RNA 逆转录和病毒 DNA 整合到宿主细胞基因组中所需的 miRNA 表达。 此外，土拨鼠肝炎转录后调控元件允许增强慢病毒载体传递的转基因的表达。该慢病毒载体还携带用于选择细胞的嘌呤霉素抗性基因。与基于鼠的 MMLV 或 MSCV 逆转录病毒系统不同，基于慢病毒的颗粒允许有效感染并将特定 miRNA 构建体整合到分化和非分裂细胞中，例如神经元和树突状细胞。
其他说明
Based on miRBase V20 Mature ID
推荐产品
有两种阴性对照可供选择：NCLMIR001 和 NCLMIR002
法律信息
MISSION is a registered trademark of Sigma-Aldrich Co. LLC
储存分类代码
12 - Non Combustible Liquids
WGK
WGK 3
闪点(F)
Not applicable
闪点(C)
Not applicable
Certificate of Analysis
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Certificate of Origin
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