MWND500

Sigma-Aldrich

14,000-500,000分子量非变性蛋白检测试剂盒

别名:
Native Marker, SDS Markers, Electrophoresis Marker, Molecular Weight Marker, Electrophoresis Markers, Moleuclar Weight Markers, Non Denaturing, non-denaturing, Molecular Weight, SDS Marker

形式

lyophilized powder

质量水平

200

储存温度

−20°C

相关类别

应用

Kit for Molecular Weights 14,000-500,000 Non-denaturing has been used as a standard for non-denaturing PAGE (polyacrylamide gel electrophoresis) analysis. It has also been used to calibrate columns for gel exclusion chromatography.

包装

The kit contains 5 vials of proteins (1 mg of each) having a molecular weight range of 14 - 545 kDa and a technical bulletin.

Proteins:
α-Lactalbumin from bovine milk
Carbonic Anhydrase from bovine erythrocytes
Albumin from chicken egg white
Albumin from bovine serum
Urease from Jack bean

重悬

Reconstitute the contents of each vial of protein marker with 1ml of water, with the exception of Urease, Catalog Number U7752. Reconstitute the vial of Urease with 0.5 ml of water and mix until the protein is in solution. Then add 0.5 ml of glycerol to the Urease solution to stabilize the protein during freeze thaw cycles. Solutions may be stored at -20 °C for future use. It is recommended that repeated freezing and thawing be avoided. Stock solutions may be dispensed into working aliquots, frozen, and then discarded after 2-3 uses. Immediately before use, thaw an aliquot of each prepared protein marker and dilute with an equal volume of sample buffer.

象形图

Exclamation markHealth hazard

警示用语:

Danger

hazcat

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

storage_class_code

10 - Combustible liquids

WGK Germany

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

分析证书

原产地证书 (CofO)

Assembly of phagocyte NADPH oxidase: A concerted binding process?
Gilda Karimi
Biochim. Biophys. Acta Gen. Subj. (2014)
Immunogenicity and Serological Cross-Reactivity of Saliva Proteins among Different Tsetse Species
Xin Zhao
PLoS ONE, e0004038-e0004038 (2015)
Oligomerization of the microtubule-associated protein tau is mediated by its N-terminal sequences: implications for normal and pathological tau action
H. Eric Feinstein
Journal of Neurochemistry (2016)
Costas Delis et al.
RNA biology, 13(1), 68-82 (2015-12-01)
We report the identification and characterization of a novel gene, AtHesperin (AtHESP) that codes for a deadenylase in Arabidopsis thaliana. The gene is under circadian clock-gene regulation and has similarity to the mammalian Nocturnin. AtHESP can efficiently degrade poly(A) substrates...
Gilda Karimi et al.
Biochimica et biophysica acta, 1840(11), 3277-3283 (2014-08-12)
The phagocyte NADPH-oxidase is a multicomponent enzyme that generates superoxide anions. It comprises a membrane redox component flavocytochrome b558 and four cytosolic proteins (p67(phox), p47(phox), p40(phox) and Rac) that must assemble to produce an active system. In this work we...
实验方案
Separation of Polyacrylic acid (PAA) 438 kDa; Polyacrylic acid (PAA) 235 kDa; Polyethylenimine (PEI) 266 kDa ; Poly(dimethyl diallyl ammonium chloride) (PIDADMACl) 204 kDa; p-Aminosalicylic acid (PAS) 7800 Da; p-Aminosalicylic acid (PAS) 257 kDa; Cationic dextran (11 kDa); Chitosan (13.4 kDa)
了解更多

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