N3393

Sigma-Aldrich

萘酚蓝黑

BioReagent, suitable for electrophoresis

别名:
氨基黑 10B, 酸性黑1, 水牛黑色 NBR
Empirical Formula (Hill Notation):
C22H14N6Na2O9S2
CAS号:
分子量:
616.49
颜色索引号:
20470
Beilstein/REAXYS Number:
5374263
EC 号:
MDL编号:
PubChem化学物质编号:
NACRES:
NA.32

质量水平

200

产品线

BioReagent

组成

Dye content, ~85%

应用

protein staining: suitable

杂质

≤10% water

适用性

suitable for electrophoresis

SMILES string

[Na+].[Na+].Nc1c(\N=N\c2ccc(cc2)[N+]([O-])=O)c(cc3cc(c(\N=N\c4ccccc4)c(O)c13)S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C22H16N6O9S2.2Na/c23-19-18-12(11-17(39(35,36)37)21(22(18)29)27-24-13-4-2-1-3-5-13)10-16(38(32,33)34)20(19)26-25-14-6-8-15(9-7-14)28(30)31;;/h1-11,29H,23H2,(H,32,33,34)(H,35,36,37);;/q;2*+1/p-2/b26-25+,27-24+;;

InChI key

AOMZHDJXSYHPKS-DROYEMJCSA-L

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应用

萘酚蓝黑已被用于蛋白质印迹膜的染色,可用于检测转移至膜的所有蛋白质。

包装

25, 100 g in glass bottle

生化/生理作用

萘酚蓝黑染料可用于在聚丙烯酰胺凝胶、琼脂糖凝胶和硝酸纤维素膜上对蛋白质进行染色。电泳后,建议将蛋白固定在凝胶中。然后可以将凝胶用0.1%萘酚蓝黑在7%(v/v)的乙酸中染色至少2小时,并用7%(v/v)乙酸溶液脱色。检测灵敏度约为考马斯蓝R的20%。

storage_class_code

13 - Non Combustible Solids

WGK Germany

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves

分析证书

原产地证书 (CofO)

Disc electrophoresis of proteins in the presence of sodium dodecyl sulphate.
E De Vito et al.
Experientia, 22(2), 124-125 (1966-02-15)
PopF1 and PopF2, two proteins secreted by the type III protein secretion system of Ralstonia solanacearum, are translocators belonging to the HrpF/NopX family.
Meyer D, et al.
Journal of Bacteriology, 188, 4903-4917 (2006)
Teresa M Lamb et al.
G3 (Bethesda, Md.), 3(12), 2273-2280 (2013-10-22)
Precise control of gene expression is a powerful method to elucidate biological function, and protein overexpression is an important tool for industry and biochemistry. Expression of the Neurospora crassa tcu-1 gene (NCU00830), encoding a high-affinity copper transporter, is tightly controlled...
M B Kasiri et al.
Environmental technology, 33(10-12), 1417-1425 (2012-08-04)
The effects of different operational parameters on the decolorization of a dye solution containing C.I. Acid Blue 92 (AB92) or C.I. Acid Black 1 (AB1) by the UV/H2O2 process were optimized using response surface methodology (RSM). The reaction time, dye...
Staining of proteins on gels: comparisons of dyes and procedures.
C M Wilson
Methods in enzymology, 91, 236-247 (1983-01-01)

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