N6663

Sigma-Aldrich

Monoclonal Anti-NUP153 antibody produced in rat

~1.0 mg/mL, clone R3G1, purified immunoglobulin, buffered aqueous solution

别名:
Anti-NUCZINK, Anti-Nucleoporin 153
NACRES:
NA.41

Quality Level

biological source

rat

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

R3G1, monoclonal

form

buffered aqueous solution

mol wt

antigen apparent mol wt ~160 kDa
antigen predicted mol wt ~153 kDa

species reactivity

primate, rat, mouse, human

packaging

antibody small pack of 25 μL

concentration

~1.0 mg/mL

application(s)

immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: 2-4 μg/mL using HeLa total cell extract

isotype

IgG2a

conjugate

unconjugated

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... NUP153(9972)
mouse ... Nup153(218210)
rat ... Nup153(25281)

General description

Nucleoporin 153kDa (NUP153) has a NE (nuclear envelope)-and NPC (nuclear pore complex)-targeting site, four zinc-fingers and a phenylalanine-glycine-repeat. It is part of the FG repeat class of proteins. The gene encoding it is localized to human chromosome 6p22.3.

Immunogen

recombinant protein corresponding to amino acids 610-1191 of rat Nup15.

Application

Monoclonal Anti-NUP153 antibody produced in rat has been used to study binding protein partners for thyroid hormone receptor-β2.

Biochem/physiol Actions

Nucleoporin 153kDa (NUP153) is a nuclear pore protein crucial for nuclear import. It is involved in importin α/β-mediated import and mRNA export from the nucleus. NUP153 is involved in HIV (human immunodeficiency virus)-1 nuclear import and integration. Poliovirus and rhinovirus infection results in the cleavage of this protein, thereby disrupting nucleo-cytoplasmic trafficking. Similarly, cardiovirus infection results in NUP153 phosphorylation, thereby modulating nuclear pore complex. NUP153 also has a role in cell cycle modulation and apoptosis. It associates with lamin B and might have a role in the anchoring of nuclear pore complexes to the nuclear lamina.

Physical form

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Personal Protective Equipment

dust mask type N95 (US),Eyeshields,Gloves

RIDADR

NONH for all modes of transport

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Gains and overexpression identify DEK and E2F3 as targets of chromosome 6p gains in retinoblastoma.
Grasemann C
Oncogene (2005)
Structural characterization of altered nucleoporin Nup153 expression in human cells by thin-section electron microscopy.
Duheron
Nucleus (Austin, Tex.), 5(6) (2014)
Nup153 and Nup98 bind the HIV-1 core and contribute to the early steps of HIV-1 replication.
Di Nunzio
Virology (2013)
Johnnie B Hahm et al.
Molecular endocrinology (Baltimore, Md.), 27(5), 840-859 (2013-04-06)
Thyroid hormone receptors (TRs) are expressed as a series of interrelated isoforms that perform distinct biological roles. The TRβ2 isoform is found predominantly in the hypothalamus, pituitary, retina, and cochlea and displays unique transcriptional properties relative to the other TR...
Research Resource: Identification of Novel Coregulators Specific for Thyroid Hormone Receptor-?2
Johnnie B
Molecular Endocrinology (2013)

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