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用途
sufficient for 25 nuclei preparations (~1-10×107 cells/preparation)
质量水平
保质期
1 yr at 2‑8 °C
包装
pkg of 1 kit
应用
cell analysis
异质活性
nuclease and protease, free
运输
wet ice
储存温度
2-8°C
一般描述
Nuclei EZ Prep Kit用于从哺乳动物细胞中快速分离胞核。该试剂盒可从通用细胞类型获得高得率细胞核。
应用
适于作为细胞核成分的来源，为体外凋亡检测和功能研究提供细胞核成分。
生化/生理作用
配套实验方案使您可从常用的哺乳动物细胞获得高得率的细胞核，包括贴壁 （例如，HEK293和COS7）和非贴壁（例如，Jurkat和HFN7.1）细胞系和外周血单核细胞(PBMC)。该制备适用于许多细胞生物学应用，例如：为染色质、基因组DNA、组蛋白和核RNA/RNP提供细胞核来源，为体外凋亡检测、功能性研究（如细胞转录状态检查）制备细胞核。
其他说明
所有操作步骤应在冰块上或2-8°C条件下进行。
相关产品
产品编号
说明
价格
储存分类代码
10 - Combustible liquids
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
Christina N Vallianatos et al.
Communications biology, 3(1), 278-278 (2020-06-03)
Histone H3 lysine 4 methylation (H3K4me) is extensively regulated by numerous writer and eraser enzymes in mammals. Nine H3K4me enzymes are associated with neurodevelopmental disorders to date, indicating their important roles in the brain. However, interplay among H3K4me enzymes during
Analysis of nuclear RNA, Chapter 14.
Robert E. Farrell, Jr., ed.
RNA Methodologies: A Laboratory Guide for Isolation and Characterization, 235-263 (1993)
Buqing Ye et al.
Nature communications, 8(1), 1518-1518 (2017-11-16)
Lymphoid lineage commitment is an important process in haematopoiesis, which forms the immune system to protect the host from pathogen invasion. However, how multipotent progenitors (MPP) switch into common lymphoid progenitors (CLP) or common myeloid progenitors (CMP) during this process
Bas Castelijns et al.
Nature communications, 11(1), 301-301 (2020-01-18)
Speciation is associated with substantial rewiring of the regulatory circuitry underlying the expression of genes. Determining which changes are relevant and underlie the emergence of the human brain or its unique susceptibility to neural disease has been challenging. Here we
Naomi Habib et al.
Nature methods, 14(10), 955-958 (2017-08-29)
Single-nucleus RNA sequencing (sNuc-seq) profiles RNA from tissues that are preserved or cannot be dissociated, but it does not provide high throughput. Here, we develop DroNc-seq: massively parallel sNuc-seq with droplet technology. We profile 39,111 nuclei from mouse and human
商品
亚细胞组分离心分离是一种常用技术，广泛适用于多种类型的细胞和组织。由于不同细胞器的大小、形状和密度不同，因此通过离心，可从温和均质的样品中轻松分离和纯化细胞器组分。
Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.
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