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OGS1486

Sigma-Aldrich

PSF-CMV-PURO-NH2-BM40-10HIS - BM40 SECRETION AND 10HIS TAG PLASMID

plasmid vector for molecular cloning

Synonym(s):
expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid
NACRES:
NA.85

标签

10-His tagged

形式

buffered aqueous solution

分子量

size 6224 bp

菌种筛选

kanamycin

哺乳动物细胞筛选

puromycin

复制起点

pUC (500 copies)

肽切割

no cleavage

肽标签位置

N-terminal

启动子

Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian

报告基因

none

分泌信号

BM40

运输

ambient

储存温度

−20°C

一般描述

This plasmid is designed to express tagged proteins in mammalian cells either by transient transfection or by creating stable cell lines. It contains a puromycin resistance expression cassette using the human Ubiquitin promoter to drive expression and allow for the selection of cells containing the plasmid.

About the Peptide Tag:This plasmid contains an Human BM-40 (osteonectin SPARC) secretory signal peptide (SP) to allow proteins to be exported from the cytosol. During translocation from the cytosol the signal peptide is removed from the protein by endogenous proteases. This plasmid also contains a secondary Deca-Histidine (10His) protein tag. The sequence of this tag is: HHHHHHHHHHWe provide a range of dual peptide tag plasmids. This is because some peptide tags provide specific biological properties (e. g., small molecule affinity new epitopes solubility or protein secretion) that are not provided by others.

Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture.

序列

Please select the file type you require. For reference most cloning programs will import a .gb (Genbank) file and will show all of the plasmids features automatically when downloaded and imported.

Genebank Vector Sequence File

FASTA Vector Sequence File

Full Plasmid Map

分析说明

To view the Certificate of Analysis for this product, please visit www.oxfordgenetics.com.

其他说明

Looking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics. Find out more at Oxford Genetics - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.

法律信息

Oxford Genetics is a trademark of Oxford Genetics Ltd

储存分类代码

12 - Non Combustible Liquids

闪点(F)

Not applicable

闪点(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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