OGS185

Sigma-Aldrich

PSF-T7-EMCV - T7 IRES EXPRESSION PLASMID

plasmid vector for molecular cloning

别名:
expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid
NACRES:
NA.85

form

buffered aqueous solution

mol wt

size 4378 bp

Origin of replication

pUC (500 copies)

Peptide cleavage

no cleavage

Promoter

Promoter name: T7
Promoter activity: inducible
Promoter type: phage

bacteria selection

kanamycin

reporter gene

none

shipped in

ambient

storage temp.

−20°C

General description

PSF-T7-EMCV - T7 IRES EXPRESSION PLASMID allows transcription of un-polyadenylated and un-capped RNA using the T7 bacteriophage polymerase. This plasmid also contains the internal ribosome entry site (IRES) from Encephalomyocarditis virus (ECMV) between the promoter and the multiple cloning site (MCS). It is designed to be used to express genes using eukaryotic T7 expression systems such as those used for the recovery of a variety of viruses. This vector is not designed for the expression of two proteins from the same mRNA. For this purpose we have designed pSF-CMV-MCS-IRES-PciI where the PciI site contains a start codon in the correct position for the IRES to express efficiently. In this vector there is an NcoI site (which contains an ATG start codon) in the correct position to enable efficient expression of a gene in the MCS.

Application

The start codon that is within the NcoI restriction site is positioned to allow efficient expression from the ECMV IRES. The main MCS for gene insertions in this molecular cloning vector therefore extends from NcoI to XbaI. Downstream sites have alternate functions and are used to insert other DNA sequences that we sell however they can be used to insert a gene if these functions are not required.

The ClaI to NheI sites have other functions such as adding peptide tags second promoters or other IRES elements. The BsgI and BseRI restriction sites cleave within the stop codon in the XbaI site and allow the retrospective fusion of coding sequences. These sites are normally only used on genes that we sell in the main multiple cloning site.

Sequence

To view sequence information for this product, please visit the product page

Analysis Note

To view the Certificate of Analysis for this product, please visit www.oxgene.com

RIDADR

NONH for all modes of transport

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

分析证书
原产地证书 (CofO)
Geoffrey M Lynn et al.
Nature biotechnology, 33(11), 1201-1210 (2015-10-27)
The efficacy of vaccine adjuvants such as Toll-like receptor agonists (TLRa) can be improved through formulation and delivery approaches. Here, we attached small molecule TLR-7/8a to polymer scaffolds (polymer-TLR-7/8a) and evaluated how different physicochemical properties of the TLR-7/8a and polymer...
Diana Romero et al.
Carcinogenesis, 37(1), 18-29 (2015-10-28)
Dickkopf-3 (Dkk-3) is a secreted protein whose expression is downregulated in many types of cancer. Endogenous Dkk-3 is required for formation of acini in 3D cultures of prostate epithelial cells, where it inhibits transforming growth factor (TGF)-β/Smad signaling. Here, we...
Jin-Gyoung Jung et al.
PLoS genetics, 10(10), e1004751-e1004751 (2014-10-31)
The Notch3 signaling pathway is thought to play a critical role in cancer development, as evidenced by the Notch3 amplification and rearrangement observed in human cancers. However, the molecular mechanism by which Notch3 signaling contributes to tumorigenesis is largely unknown....
Alexander C Cerny et al.
PLoS genetics, 11(10), e1005578-e1005578 (2015-10-29)
Recycling of signaling proteins is a common phenomenon in diverse signaling pathways. In photoreceptors of Drosophila, light absorption by rhodopsin triggers a phospholipase Cβ-mediated opening of the ion channels transient receptor potential (TRP) and TRP-like (TRPL) and generates the visual...

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