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OGS2725

Sigma-Aldrich

PSF-OXB20-COOH-THR-6HIS - C-TERMINAL 6 HIS TAG BACTERIAL PLASMID

plasmid vector for molecular cloning

Synonym(s):
expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid

重组

expressed in E. coli

标签

6-His tagged

形式

buffered aqueous solution

分子量

size 3890 bp

菌种筛选

kanamycin

复制起点

pUC (500 copies)

肽切割

thrombin

肽标签位置

C-terminal

启动子

Promoter name: OXB20
Promoter activity: constitutive
Promoter type: bacterial

报告基因

none

运输

ambient

储存温度

−20°C

一般描述

This plasmid is designed to express tagged proteins in E. coli. The plasmid contains a constitutive promoter (OXB20) derived from the region upstream of the E. coli RecA gene. It does not require induction or any additional components for activity. It is the strongest of the bacterial promoters that we provide and this high level of expression can cause expression problems with some proteins with poor solubility. For this reason we sell a range of bacterial promoters with different expression levels (OXB1(low)>OXB20(high)) that can be provided with the peptide tags in this plasmid on request.

About the Peptide Tag:This plasmid contains a c-terminal Hexa-Histidine (6His) affinity tag that can be fused to a gene of interest to allow protein detection and/or purification. The sequence of the tag is: HHHHHH.

About the Cleavage Tag:This plasmid also encodes a protease cleavage site that is designed to be positioned between your gene of interest and the tag to allow the removal of the tag following protein purification or isolation. This plasmid contains a Thrombin cleavage tag. The protein sequence of the cleavage tag is: LVPRGS. It cleaves preferentially between the Arg and Gly residues. Off target cleavage can often occur at non-specific sites normally from other contaminating proteases. To ensure maximal protein integrity the enzyme reagent must be highly pure.

Promoter Expression Level: This plasmid contains a constitutive bacterial promoter that does not require induction. It is the strongest bacterial promoter we sell and this can cause solubility and expression problems with some proteins. We also offer a range of other bacterial promoters that are compatible with this plasmid and are available on request.

序列

Please select the file type you require. For reference most cloning programs will import a .gb (Genbank) file and will show all of the plasmids features automatically when downloaded and imported.

Genebank Vector Sequence File

FASTA Vector Sequence File

Full Plasmid Map

分析说明

To view the Certificate of Analysis for this product, please visit www.oxfordgenetics.com.

其他说明

Looking for more vector options to move your experiments forward faster? Consider a custom cloning vector designed and built by Oxford Genetics. Find out more at Oxford Genetics - Sigma's partner for cloning and expression vectors for molecular biology and synthetic biology applications.

法律信息

Oxford Genetics is a trademark of Oxford Genetics Ltd

储存分类代码

12 - Non Combustible Liquids

闪点(F)

Not applicable

闪点(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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