OGS595

Sigma-Aldrich

PSF-RENILLA-PHOTINUS - BI-DIRECTIONAL FIREFLY RENILLA LUCIFERASE PLASMID

plasmid vector for molecular cloning

别名:
expression vector, molecular cloning vector, vector, snapfast vector, plasmid vector, cloning vector, plasmid
NACRES:
NA.85

形式

buffered aqueous solution

分子量

size 6170 bp

复制起点

pUC (500 copies)

肽切割

no cleavage

菌种筛选

kanamycin

报告基因

Renilla luciferase

运输

ambient

储存温度

−20°C

一般描述

This plasmid has been designed so that sequences can inserted between two reporter genes (renilla and firefly luciferase) to allow the relative transcription level in both directions to be determined by measuring the reporter luminescence. Dual luciferase reporter assay kits are available from other providers that are compatible with this plasmid. The plasmid only contains a Bgl2 site between the two genes to allow insertion.

Promoter Expression Level:

应用

This plasmid contains two luciferase reporter genes (Firefly and Renilla) in opposing directions with a Bgl2 restriction site in between the two genes to allow promoters to be inserted. This allows the transcriptional level of DNA sequences and promoters to be determined in both directions by inserting the sequences into the Bgl2 restriction site. If the sequence you would like to insert contains a Bgl2 site fragments with BamHI also have the same overhangs and can be ligated into this plasmid cut with Bgl2.

序列

To view sequence information for this product, please visit the product page

分析说明

To view the Certificate of Analysis for this product, please visit www.oxgene.com

storage_class_code

12 - Non Combustible Liquids

闪点(F)

Not applicable

闪点(C)

Not applicable

分析证书

原产地证书 (CofO)

Michael Craigie et al.
Journal of neuroimmune pharmacology : the official journal of the Society on NeuroImmune Pharmacology, 13(2), 126-142 (2017-11-22)
JC virus (JCV) is a human polyomavirus and the etiologic agent of the demyelinating disease progressive multifocal leukoencephalopathy (PML). PML is observed in patients with underlying immunocompromising conditions, suggesting that neuro-immune interactions between peripheral immune cells and neuro-glia play an...
Jin-Gyoung Jung et al.
PLoS genetics, 10(10), e1004751-e1004751 (2014-10-31)
The Notch3 signaling pathway is thought to play a critical role in cancer development, as evidenced by the Notch3 amplification and rearrangement observed in human cancers. However, the molecular mechanism by which Notch3 signaling contributes to tumorigenesis is largely unknown....
Alexander C Cerny et al.
PLoS genetics, 11(10), e1005578-e1005578 (2015-10-29)
Recycling of signaling proteins is a common phenomenon in diverse signaling pathways. In photoreceptors of Drosophila, light absorption by rhodopsin triggers a phospholipase Cβ-mediated opening of the ion channels transient receptor potential (TRP) and TRP-like (TRPL) and generates the visual...
Diana Romero et al.
Carcinogenesis, 37(1), 18-29 (2015-10-28)
Dickkopf-3 (Dkk-3) is a secreted protein whose expression is downregulated in many types of cancer. Endogenous Dkk-3 is required for formation of acini in 3D cultures of prostate epithelial cells, where it inhibits transforming growth factor (TGF)-β/Smad signaling. Here, we...
Geoffrey M Lynn et al.
Nature biotechnology, 33(11), 1201-1210 (2015-10-27)
The efficacy of vaccine adjuvants such as Toll-like receptor agonists (TLRa) can be improved through formulation and delivery approaches. Here, we attached small molecule TLR-7/8a to polymer scaffolds (polymer-TLR-7/8a) and evaluated how different physicochemical properties of the TLR-7/8a and polymer...

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