Merck

P0294

Sigma-Aldrich

丙酮酸激酶/乳酸脱氢酶 来源于兔肌肉

For the Determination of ADP, buffered aqueous glycerol solution

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别名:
PK/LDH酶 来源于兔肌肉
NACRES:
NA.54

质量水平

形式

buffered aqueous glycerol solution

分子量

59 kDa

浓度

600-1,000 units/mL pyruvate kinase
900-1400 units/mL lactic dehydrogenase

储存温度

−20°C

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此商品
10109045001SRP0189550601
Pyruvate Kinase (PK) from rabbit muscle

Roche

10109045001

丙酮酸激酶 (PK)

Pyruvate Kinase Activator The Pyruvate Kinase Activator controls the biological activity of Pyruvate Kinase. This small molecule/inhibitor is primarily used for Activators/Inducers applications.

Sigma-Aldrich

550601

Pyruvate Kinase Activator

mol wt

59 kDa

mol wt

-

mol wt

58 kDa

mol wt

-

concentration

600-1,000 units/mL pyruvate kinase, 900-1400 units/mL lactic dehydrogenase

concentration

-

concentration

>0.02 mg/mL

concentration

-

storage temp.

−20°C

storage temp.

−20°C (−15°C to −25°C)

storage temp.

−70°C

storage temp.

2-8°C

一般描述

兔肌丙酮酸激酶是一种金属酶,可用于在糖酵解途径中催化磷酸烯醇丙酮酸转化为丙酮酸。它的分子量为59 kDa。它以四聚体的形式存在,其活性部位发生构型变化以适应底物。乳酸脱氢酶(LDH)在无氧糖酵解中催化乳酸转化为丙酮酸。它以四聚体的形式存在,由两个亚基(H和M)组成。真核生物的LDH通过活性位点循环门控实现催化功能。

应用

兔肌来源丙酮酸激酶/乳酸脱氢酶可用于:
  • 在活性微管制备中产生ATP
  • 骨骼肌重酶解肌球蛋白(HMM)的酶联ATP酶测定
  • 作为量化间充质干细胞(MSC)乳酸脱氢酶的标准对照品。

生化/生理作用

在测定ADP中使用PK/LDH的ADP定量检测方案。 在该方案中,含有未知浓度ADP的溶液可代替试剂D。可能需要进一步稀释ADP溶液
丙酮酸激酶进行活化分别需要二价和一价阳离子(如Mg2+和K+)。
抗坏血酸可抑制兔肌肉乳酸脱氢酶。醛缩酶和肌动蛋白被证明可以阻断这种抑制作用。
此外,丙酮酸激酶还可催化二磷酸硫胺磷酸化(TDP)为三磷酸硫胺素(TTP),可应用于抗病毒和肿瘤治疗。

单位定义

丙酮酸激酶活性:在pH7.6,37℃下,一单位酶每分钟可将1.0 μmole的磷酸(烯醇)丙酮酸转化为丙酮酸。
乳酸脱氢酶活性:在pH7.5,37℃下,一单位酶每分钟可将1.0 μmole丙酮酸盐还原为L-乳酸盐。

外形

含有10mM HEPES,pH 7.0,100mM KCl和0.1mM EDTA的50%甘油溶液

储存分类代码

10 - Combustible liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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