所有图片(3)

P0609

Sigma-Aldrich

胃蛋白酶−琼脂糖 来源于猪胃粘膜

lyophilized powder, ≥30 units/mg dry solid

别名:
胃蛋白酶 A
MDL编号:
NACRES:
NA.54

形式

lyophilized powder

质量水平

200

specific activity

≥30 units/mg dry solid

分子量

35 kDa

基质

4% cross-linked beaded agarose

UniProt登记号

储存温度

−20°C

Gene Information

一般描述

胃蛋白酶是一种在胃液分泌物中发现的酶。

应用

固定在琼脂糖珠上的胃蛋白酶可用于消化并随后从糖蛋白麦醇溶蛋白中分离出活性肽。
胃蛋白酶-琼脂糖已作为固定工具用于研究胃蛋白酶的重折叠能力。它还用于从兔IgG制备F(ab′)2片段。
用于产生抗体的F(ab′)2 片段。1

包装

10000, 25000, 50000 units in poly bottle

生化/生理作用

优先将C末端切割成Phe、Leu和Glu。它不会在Val、Ala或Gly上进行切割。最佳pH2-4。活性成分为4 M尿素和3 M盐酸胍。在60°C时稳定。胃蛋白酶在pH > 6时会不可逆地失活。

单位定义

在pH2.0,37℃条件下用血红蛋白作为底物,对TCA可溶性产物进行测定时,一个单位每分钟可产生0.001 ΔA的280(终体积 = 16 mL。光程 = 1 cm。)

数量

一克固体可膨胀至5-10ml填充凝胶。

外形

用乳糖稳定化的冻干粉

象形图

Health hazard

警示用语:

Danger

危险声明

危险分类

Resp. Sens. 1

储存分类代码

13 - Non Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

分析证书

原产地证书 (CofO)

Ludmila Tucková et al.
Journal of leukocyte biology, 71(4), 625-631 (2002-04-03)
Celiac disease, induced by dietary gluten, is characterized by mucosal atrophy and local inflammation associated with cell infiltration and activation. Unlike other food proteins, gluten and its proteolytic fragments, besides inducing a specific immune response, were shown to activate components
Thomas A Kraus et al.
The Journal of clinical investigation, 115(8), 2234-2243 (2005-07-26)
To explore the requirement for M cells and the Peyer's patch (PP) in induction of oral tolerance and address the potential in vivo role of intestinal epithelial cells as nonprofessional APCs, we have attempted to induce tolerance in mice with
M Feldman et al.
Gastroenterology, 110(4), 1043-1052 (1996-04-01)
Recent studies suggesting that gastric secretion does not decrease with aging included few elderly individuals and measured only acid secretion. The aims of this study were to measure gastric acid and pepsin output in 206 health Americans (age range, 18-98
Hana Dvořáková et al.
Frontiers in cellular and infection microbiology, 10, 66-66 (2020-03-17)
Schistosomula (the post-infective stages) of the neurotropic schistosome Trichobilharzia regenti possess multiple isoforms of cathepsin B1 peptidase (TrCB1.1-TrCB1.6) with involvement in nutrient digestion. The comparison of substrate preferences of TrCB1.1 and TrCB1.4 showed that TrCB1.4 had a very narrow substrate
Mahmoud S Ali et al.
The Laryngoscope, 123(2), 434-439 (2012-10-17)
To assess if, as previously reported in the literature, bile acids inhibit pepsin activity, resulting in pepsin having a less important role in laryngopharyngeal damage in reflux disease. Prospective translational research study. A total of 78 patient's fasting gastric juice

实验方案

Enzymatic Assay of Pepsin (3.4.23.1)

This procedure may be used for determination of Pepsin activity using hemoglobin as the substrate. It is a spectrophotometric stop rate determination.

相关内容

胃蛋白酶的酶法测定(3.4.23.1)

本方法可用于以血红蛋白为底物的胃蛋白酶活性测定。这是一种分光光度速率终止测定法。

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门