Merck
All Photos(4)

P1524

Sigma-Aldrich

聚-L-赖氨酸 氢溴酸盐

mol wt ≥300,000

CAS Number:
MDL编号:
eCl@ss:
32160406
PubChem化学物质编号:
NACRES:
NA.26

形式

powder

质量水平

200

分子量

≥300,000

technique(s)

cell culture | mammalian: suitable

颜色

white to off-white

application(s)

cell analysis

储存温度

−20°C

SMILES string

Cl.NCCCCC(N)C(O)=O

InChI

1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1

InChI key

WBSCNDJQPKSPII-KKUMJFAQSA-N

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应用

多聚赖氨酸聚合物可用于促进细胞对固体底物的黏附、与甲氨蝶呤结合增加药物转运、胰岛的微囊化、细胞微囊化技术、微阵列载玻片包被和染色体制备等。它也被用作高效标记 和促进胞吞作用的转染剂。低分子量的多聚-L-赖氨酸 (30,000-70,000) 在溶液中粘度较低,但高分子量的版本提供了更多的每个分子的附着位点。

包装

25, 100, 500 mg in poly bottle
1 g in poly bottle

生化/生理作用

多聚赖氨酸是一种非特异性的粘附因子,通过增强细胞膜和培养表面带负电荷离子之间的静电作用,促进细胞与固体基质的粘附。当它被吸收到细胞培养表面时,多聚左旋赖氨酸的功能是增加细胞结合可用的带正电荷位点的编号。对于能消化多聚-L-赖氨酸的细胞,应以多聚-D-赖氨酸作为附着因子。

组分

聚-L-赖氨酸是一种带正电荷的氨基酸聚合物,每个赖氨酸残基约有一个HBr。 氢溴酸盐可使聚-L-赖氨酸呈可溶于水的结晶形式。 在β结构中可能会发现少量的产物,因为HBr干扰氨基与羧基或含N或O部分之间的氢键。

注意

无菌溶液可在2-8℃条件下稳定保存2年。脱水保存在-20°C条件下。

制备说明

聚-L-赖氨酸氢溴酸盐的分子量> 300,000。为除去 HBr,将本品溶于中性缓冲液中,透析除去盐类。未将聚-L-赖氨酸产品暴露于三氟乙酸,并通过薄层色谱法确认透析除去任何单体、二聚体或三聚体。一般以本品为附着因子,在 5 mg 聚赖氨酸中加入 50 mL 无菌组织培养级水,每 25 cm2 溶液无菌涂布表面 1 mL。5 min 后,通过抽吸清除溶液,并彻底冲洗表面。在引入细胞和培养基前干燥两小时。

分析说明

分子量基于粘度,也由 MALLS 测定。

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

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