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一般描述
纤溶酶作为纤维蛋白溶解级联的关键酶起作用，并且在炎症过程中也是重要的。
应用
通过来自尿激酶活化的人血浆的亲和色谱法在研究中分离了纤溶酶和抑制剂之间的复合物。 它还被用于研究纤溶酶和胰凝乳蛋白酶激活人上皮钠通道（ENaC）的研究。
生化/生理作用
它会被α2-抗纤溶酶抑制并且阻断其与纤维蛋白的相互作用。 它消化细胞质蛋白α突触核蛋白的N-末端区域，防止其被周围细胞摄取。
纤溶酶在赖氨酸和精氨酸残基的羧基侧表现出优先的切割，其选择性高于胰蛋白酶。它可将聚合纤维蛋白转化为可溶性产物。
最适 pH：8.5
pH 7.5：约 40％ 的最大活性，约 50％ 的最大活性
最适温度：37°C，于 56°C 快速失活
最适 pH：8.5
pH 7.5：约 40％ 的最大活性，约 50％ 的最大活性
最适温度：37°C，于 56°C 快速失活
包装
包装尺寸基于蛋白质含量
单位定义
一个单元将在 pH7.5，37℃ 下，每分钟从 D-Val-Leu-Lys-p-硝基苯胺生产 μmole 对硝基苯胺。
外形
含有磷酸钠、甘露醇和 NaCl 的冻干粉末。
免责声明
仅供研究使用。在法国，在用于科研目的时（包括进口和出口活动（《公共健康法》第L 1211-1条，第2节））该产品为管制品。购买者（即最终用户）需要获得《公共健康法》法第L 1211-1条规定的法国研究部的进口授权。订购本产品即表明您确认已经获得合适的进口授权。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
其他客户在看
Silke Haerteis et al.
The Journal of general physiology, 140(4), 375-389 (2012-09-12)
Proteolytic activation of the epithelial sodium channel (ENaC) involves cleavage of its γ subunit in a critical region targeted by several proteases. Our aim was to identify cleavage sites in this region that are functionally important for activation of human
S Müllertz et al.
The Biochemical journal, 159(3), 545-553 (1976-12-01)
A complex between plasmin and an inhibitor was isolated by affinity chromatography from urokinase-activated human plasma. The complex did not react with antibodies against any of the known proteinase inhibitors in plasma. A rabbit antiserum against the complex was produced.
Tatiana Syrovets et al.
Journal of leukocyte biology, 92(3), 509-519 (2012-05-09)
The serine protease plasmin generated from its zymogen plasminogen is best known for its function as a key enzyme of the fibrinolytic cascade. However, beyond fibrinolysis, plasmin has a number of crucial functions in a variety of processes, including inflammation.
Efrat Shavit-Stein et al.
PloS one, 16(3), e0248431-e0248431 (2021-03-16)
Ischemic stroke is a common and debilitating disease with limited treatment options. Protease activated receptor 1 (PAR1) is a fundamental cell signaling mediator in the central nervous system (CNS). It can be activated by many proteases including thrombin and plasmin
On the mechanism of the reaction between human alpha 2-antiplasmin and plasmin.
B Wiman et al.
The Journal of biological chemistry, 254(18), 9291-9297 (1979-09-25)
实验方案
Protocol for Enzymatic Assay of Plasmin with D-Val-Leu-Lys-p-Nitroanilide Dihydrochloride
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