Merck
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文件

P2165

Sigma-Aldrich

蛋白A-生物素 来源于金黄色葡萄球菌

lyophilized powder

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MDL编号:
MFCD00132098
NACRES:
NA.46

偶联物

biotin conjugate

质量水平

200
300

形式

lyophilized powder

组成

Protein, ≥70% biuret

标记范围

3.0-8.0 mol biotin per mol Protein A

储存温度

2-8°C

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此商品
P8651P6031S2014
Sigma-Aldrich

Sigma-Aldrich

P2165

蛋白A-生物素 来源于金黄色葡萄球菌

Sigma-Aldrich

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P8651

蛋白A-过氧物酶 来源于金黄色酿脓葡萄球菌/辣根

Protein A from Staphylococcus aureus Soluble, essentially salt-free, lyophilized powder, extracellular

Sigma-Aldrich

P6031

蛋白质A 来源于金黄色葡萄球菌

Sigma-Aldrich

Sigma-Aldrich

S2014

Staphylococcus aureus

form

lyophilized powder

form

lyophilized powder

form

essentially salt-free, lyophilized powder

form

buffered aqueous suspension

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

300

Quality Level

100, 200

Quality Level

200

Quality Level

-

composition

Protein, ≥70% biuret

composition

-

composition

-

composition

Cell suspension, ~10% wet weight/volume

extent of labeling

3.0-8.0 mol biotin per mol Protein A

extent of labeling

-

extent of labeling

-

extent of labeling

-

一般描述

蛋白A（SpA）是由金黄色葡萄球菌产生的42kDa毒力因子，由5个高度同源的连续胞外Ig结合结构域组成，即结构域E、D、A、B和C。该蛋白存在于分泌的和膜相关形式。

特异性

仅与大多数哺乳动物的IgG结合，除了大鼠、山羊和绵羊。

生化/生理作用

蛋白A（SpA）通过与抗体的Fc区和B细胞受体的Fab区结合而在体外引起B细胞死亡，从而抑制其调理吞噬作用。该蛋白被认为与金黄色葡萄球菌逃避宿主免疫反应有关，因此可能是预防严重金黄色葡萄球菌感染的疫苗开发方法的候选者。金黄色葡萄球菌负责医院感染以及各种人类感染，例如食物中毒，中毒性休克综合征，心内膜炎，败血病，皮肤感染，软组织感染和骨骼感染。它还负责牛和绵羊的乳腺炎。蛋白A基因可变重复区的SpA分型或DNA序列分析可用于从流行病学上不重要的菌株中确定金黄色葡萄球菌暴发菌株。

外形

含柠檬酸钠的冻干粉

制备说明

由P 6031制备，通过氨基己酰基间隔基通过酰胺键与生物素偶联。

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除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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M Balasch et al.
Journal of comparative pathology, 121(2), 139-148 (1999-07-16)
This report describes the experimental inoculation of conventional pigs with a tissue homogenate obtained from two pigs affected with postweaning multisystemic wasting syndrome (PMWS). Eight 2-month-old pigs were inoculated by the intranasal route, and two pigs were left as uninfected
Anil K Pal et al.
Biosensors, 10(10) (2020-09-30)
We report a novel self-referenced diffraction-based leaky waveguide (LW) comprising a thin (~2 µm) film of a photofunctionalisable hydrogel created by covalent attachment of a biotinylated photocleavable linker to chitosan. Streptavidin attached to the chitosan via the photocleavable linker was
spa typing method for discriminating among Staphylococcus aureus isolates: implications for use of a single marker to detect genetic micro- and macrovariation.
Koreen L et al
Journal of Clinical Microbiology, 42(2), 792-799 (2004)
Regina Stoltenburg et al.
International journal of molecular sciences, 19(2) (2018-03-03)
New, as yet undiscovered aptamers for Protein A were identified by applying next generation sequencing (NGS) to a previously selected aptamer pool. This pool was obtained in a classical SELEX (Systematic Evolution of Ligands by EXponential enrichment) experiment using the
Scott D Kobayashi et al.
mBio, 4(5), e00764-e00713 (2013-10-03)
Staphylococcus aureus is a prominent cause of human infections worldwide and is notorious for its ability to acquire resistance to antibiotics. Methicillin-resistant S. aureus (MRSA), in particular, is endemic in hospitals and is the most frequent cause of community-associated bacterial
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