P2318

Sigma-Aldrich

抗 假单胞菌外毒素 A 兔抗

whole antiserum

MDL number:
NACRES:
NA.46

biological source

rabbit

Quality Level

antibody form

whole antiserum

antibody product type

primary antibodies

clone

polyclonal

contains

15 mM sodium azide

species reactivity

(Pseudomonas aeruginosa)

application(s)

dot blot: 1:20,000
indirect ELISA: 1:250,000

conjugate

unconjugated

shipped in

dry ice

storage temp.

−20°C

General description

可与假单胞菌外毒素A发生反应,但对葡萄球菌肠毒素A、霍乱毒素和葡萄球菌肠毒素B无反应。该产品尚未检测过对活性假单胞菌外毒素A的中和效力。

Immunogen

来源于铜绿假单胞菌的外毒素A。

Application

兔抗假单胞菌外毒素A抗体可用于:
  • 血清融合毒素的体外稳定性分析
  • 构建靶向表皮生长因子受体(EGFR)的毒素,以研究其对转染人EGFR的Swiss小鼠胚胎NIH-3T3细胞的毒性
  • 测定血清中免疫毒素的体内半衰期

抗假单胞菌外毒素A抗体可用于融合毒素的体外血清稳定性和靶向毒素的结合研究。

Biochem/physiol Actions

铜绿假单胞菌外毒素A由铜绿假单胞菌分泌,具有较强的细胞毒性作用。外毒素A通过受体介导的内吞作用进入细胞,转运到细胞质,通过ADP核糖化因子2抑制蛋白质合成。嵌合外毒素A在各种疾病的基因治疗和可能的癌症治疗中都有应用。

Physical form

本产品是含有15 mM 叠氮化钠防腐剂的液体。

Storage and Stability

如需连续使用,可在2-8 °C保存至多一月。对于更长期的存储,可对溶液进行等分并冷冻保存。不建议反复冻融。不建议在无霜冰箱中储存。如果长期储存后发生轻微浑浊,请在使用前通过离心澄清溶液。

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

RIDADR

NONH for all modes of transport

WGK Germany

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Fusion of an albumin-binding domain extends the half-life of immunotoxins
Guo R, et al.
International Journal of Pharmaceutics, 511(1), 538-549 (2016)
Alexander Weng et al.
Molecular oncology, 6(3), 323-332 (2012-02-09)
Tumor-targeting protein toxins are composed of a toxic enzyme coupled to a specific cell binding domain that targets cancer-associated antigens. The anti-tumor treatment by targeted toxins is accompanied by dose-limiting side effects. The future prospects of targeted toxins for therapeutic...
Characterization of an HLA-restricted and human cytomegalovirus-specific antibody repertoire with therapeutic potential.
Bewarder, et al.
Cancer Immunology, Immunotherapy, 69, 1535-1548 (2020)
Alexander N Wein et al.
Journal of medicinal chemistry, 55(18), 7998-8006 (2012-09-08)
Protective antigen (PA), lethal factor, and edema factor, the protein toxins of Bacillus anthracis , are among its most important virulence factors and play a key role in infection. We performed a virtual ligand screen of a library of 10000...
Yi Hu et al.
Leukemia research, 35(9), 1254-1260 (2011-02-08)
We have previously demonstrated that CCR9 plays a pivotal role in drug resistance and invasion in human acute T-lymphocytic leukemia (T-ALL). In this study, we investigated whether the MOLT4 cells, which naturally express CCR9 at high levels, can be successfully...

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