Merck
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文件

P3391

Millipore

蛋白 A-琼脂糖™ 来源于金黄色葡萄球菌

lyophilized powder

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别名:
蛋白 A-琼脂糖
MDL编号:
MFCD00165563
NACRES:
NA.56

形式

lyophilized powder

质量水平

200

包装

glass bottle of 1 g
glass bottle of 1.5 g
glass bottle of 250 mg
glass bottle of 5 g

储存条件

do not freeze

标记范围

3 mg per mL

technique(s)

affinity chromatography: suitable

颜色

white to gray

基质

Sepharose CL-4B

基质活化

cyanogen bromide

基质附着

amino

基质隔离区

1 atom

容量

≥16 mg/mL binding capacity (human IgG)

溶解性

water: 1 g/L at 20 °C

适用性

conforms to structure for swelling capacity (1g swells to 4 to 5 mL)

储存温度

2-8°C

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此商品
P942482526P3296
Millipore

Millipore

P3391

蛋白 A-琼脂糖™ 来源于金黄色葡萄球菌

Millipore

Millipore

P9424

ProteinA-Sepharose™ 4, Fast Flow 来源于金黄色葡萄球菌

Sigma-Aldrich

Sigma-Aldrich

82526

Protein A soluble from Staphylococcus aureus (Cowan strain)

Millipore

Millipore

P3296

蛋白G琼脂糖凝胶™，快速流动

packaging

glass bottle of 1 g, glass bottle of 1.5 g, glass bottle of 250 mg, glass bottle of 5 g

packaging

-

packaging

-

packaging

-

storage condition

do not freeze

storage condition

-

storage condition

-

storage condition

-

technique(s)

affinity chromatography: suitable

technique(s)

affinity chromatography: suitable

technique(s)

ELISA: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

technique(s)

affinity chromatography: suitable

matrix

Sepharose CL-4B

matrix

Sepharose 4B Fast Flow

matrix

-

matrix

Sepharose 4B Fast Flow

solubility

water: 1 g/L at 20 °C

solubility

-

solubility

H2O: 1 mg/mL, clear, colorless

solubility

-

一般描述

当与抗体连接时，蛋白A-琼脂糖凝胶有助于提高交联到柱基质上的IgG的抗原结合能力。在这种情况下，结合发生在蛋白A与IgG分子的Fc部分之间，使抗原特异性位点游离。

应用

蛋白A-琼脂糖凝胶™用于亲和层析、抗体纯化和表征、免疫亲和基质、蛋白层析、蛋白A、G和L树脂以及重组蛋白表达和分析。蛋白A-琼脂糖凝胶™已用于制定研究蛋白相互作用的策略，改善乳糜泻检测，以及研究儿童糖尿病。
蛋白A-琼脂糖凝胶具有以下用途：
  • 用于免疫沉淀胎盘，使用蛋白印迹技术进行蛋白质和RNA分析。
  • 用于染色质免疫沉淀，以了解介体和特定启动子染色质结构重构之间的联系。
  • 用作裂解缓冲液，以去除雌性大鼠脑样本蛋白质提取中的内源性G型Ig。
  • 用于兔抗血清的[32P]磷肉豆蔻素C的免疫沉淀反应，以研究蛋白激酶C （PKC）和磷肉豆蔻素C （PMC）在T细胞发育过程中的下调。

数量

膨胀：1 g膨胀至约4 ml。

外形

以用乳糖和葡聚糖稳定的冻干粉形式供应。

法律信息

Sepharose is a trademark of Cytiva

相关产品

产品编号
说明
价格

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

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其他客户在看

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82492

蛋白质 A，固定在琼脂糖 CL-4B 上， 来源于金黄色葡萄球菌

Millipore

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P3351

Protein L–Agarose from Peptostreptococcus magnus

Protein A from Staphylococcus aureus Soluble, essentially salt-free, lyophilized powder, extracellular

Sigma-Aldrich

P6031

蛋白质A 来源于金黄色葡萄球菌

Protein G–Agarose lyophilized powder, Contains lactose stabilizers that must be removed prior to use.

Millipore

P7700

蛋白G-琼脂糖

Frédéric Sorgeloos et al.
Journal of virology, 85(7), 3690-3694 (2011-01-14)
The L protein encoded by Theiler's murine encephalomyelitis virus (TMEV) is a unique example of a picornaviral protein encoded by an alternative open reading frame. This protein is an important determinant of TMEV persistence in the mouse central nervous system.
P Hornbeck et al.
Molecular and cellular biology, 9(9), 3727-3735 (1989-09-01)
The regulation and expression of protein kinase C (PKC) and phosphomyristin C (PMC) (a principal substrate of PKC which is the major myristylated protein in lymphocyte and glioma lines that express it) in murine B and T lymphocytes were investigated.
M Iezzi et al.
Molecular endocrinology (Baltimore, Md.), 13(2), 202-212 (1999-02-11)
Insulin-secreting cells express four GTPases of the Rab3 family. After separation of extracts of INS-1 cells on a sucrose density gradient, the bulk of the A, B, and C isoforms was recovered in the fractions enriched in insulin-containing secretory granules.
Tracie A Hennen-Bierwagen et al.
Plant physiology, 149(3), 1541-1559 (2009-01-27)
Starch biosynthetic enzymes from maize (Zea mays) and wheat (Triticum aestivum) amyloplasts exist in cell extracts in high molecular weight complexes; however, the nature of those assemblies remains to be defined. This study tested the interdependence of the maize enzymes
V Ossipow et al.
Proceedings of the National Academy of Sciences of the United States of America, 90(17), 8219-8223 (1993-09-01)
The CCAAT/enhancer-binding protein (C/EBP) alpha is a leucine zipper protein that is preferentially expressed in certain cell types, such as adipocytes and hepatocytes. Here we show that C/EBP alpha mRNA is translated into two major proteins, C/EBP-42 and C/EBP-30, that
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