Merck

P3563

Sigma-Aldrich

磷酸盐缓冲剂,含0.05% TWEEN® 20,pH 7.4

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别名:
1X PBS + 0.05% TWEEN® 20, 1X PBS + 0.05% TWEEN® 20, PBS-T, PBST
eCl@ss:
32129211
NACRES:
NA.25

形式

powder

溶解性

water: soluble, clear, colorless (1 pouch dissolved in 1L)

application(s)

diagnostic assay manufacturing

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此商品
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Phosphate buffered saline

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Phosphate buffered saline

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P5368

Phosphate buffered saline

Premium Grade
solubility

water: soluble, clear, colorless (1 pouch dissolved in 1L)

solubility

water: soluble

solubility

water: soluble

solubility

water: 9.07-10.0 g/L, clear, colorless

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

一般描述

Phosphate Buffered Saline with Tween®-20, or PBST, is primarily used in immunoassays as a washing and blocking buffer. The phosphate buffer maintains a stable pH 7.4, suitable for many biological reactions and assays. The salt or saline solution in PBS-T provides the necessary ionic strength for optimal protein solubility and stability. The addition of 0.05% TWEEN®-20, a non-ionic detergent, helps reduce background signals by blocking non-specific binding sites on proteins. PBST buffer enhances the washing efficiency by removing unwanted substances and limits non-specific antibody binding sites, while preserving the integrity of the target proteins.

应用

磷酸盐缓冲盐水已被用于:
  • 酶联免疫吸附测定(ELISA)
  • 端粒-FISH(Tel-FISH)和端粒/免疫染色-FISH(Teli-Fish)
  • 细胞分离

包装

锡箔袋包装

重悬

Contents of one pouch dissolved in 1 L dH2O or ultrapure water to yield 0.01 M phosphate buffered saline, 0.138M NaCl, 0.0027M KCl, and 0.05% Tween 20, pH7.4.

法律信息

TWEEN is a registered trademark of Croda International PLC

储存分类代码

13 - Non Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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T1503
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25G
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实验方案

ELISA实验方案提供了进行间接ELISA和捕获ELISA的详细说明。了解进行夹心式ELISA和间接ELISA实验的推荐产品和技术信息。

ELISA protocols providing detailed instructions on performing indirect ELISA and Capture ELISA . Learn more about recommended products and techniques for performing both sandwich ELISA and indirect ELISA experiments.

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