P3910

Sigma-Aldrich

蛋白酶 来源于地衣芽孢杆菌

lyophilized powder, for use in Total Dietary Fiber Assay, TDF-100A

别名:
Subtilisin Carslberg, 蛋白酶 来源于地衣芽孢杆菌, Subtilisin A, 枯草杆菌蛋白酶A
CAS号:
EC 号:
MDL编号:
eCl@ss:
32160410
NACRES:
NA.84

形式

lyophilized powder

质量水平

100

质量

for use in Total Dietary Fiber Assay, TDF-100A

分子量

27 kDa

特色行业

Food and Beverages

运输

wet ice

储存温度

2-8°C

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相关类别

特异性

枯草杆菌蛋白酶A是丝氨酸S8内蛋白酶家族的一员。它具有宽泛的特异性并在P1位对大的未带点残基具有偏好性。它可水解天然和变性的蛋白,并在碱性条件下具有活性。

应用

这是从地衣芽孢杆菌发酵中分离得到的蛋白水解酶。它是一种对天然和变性蛋白具有广泛特异性的丝氨酸内切蛋白酶,并在碱性条件下具有活性。它用于总膳食纤维检测(TDF-100A)中。

包装

500 mg in poly bottle

生化/生理作用

蛋白酶可通过肽键的水解使蛋白质分解代谢。 蛋白酶可被丝氨酸活性位点抑制剂所灭活,如苯甲基磺酰氟(PMSF)和二异丙基氟磷酸盐。

物理属性

枯草杆菌蛋白酶是一种具有27 KDa分子量并不含有二硫键的非糖基化单一多肽链。

单位定义

在pH 7.5、37°C条件下,一个单位每分钟可水解酪蛋白,生成相当于1.0 μmole (181 μg)酪氨酸的颜色。

警示用语:

Danger

hazcat

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

storage_class_code

10 - Combustible liquids

WGK Germany

WGK 1

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

分析证书

原产地证书 (CofO)

Laura Domínguez Díaz et al.
Nutrients, 12(5) (2020-05-18)
In Europe, nutrition and health claims made on food must be based on scientific evidence, which means a comprehensive evaluation by the European Food Safety Authority (EFSA) prior to authorisation. Processed foods are subject to numerous claims derived from the...
Lisete Paiva et al.
Marine drugs, 16(8) (2018-07-28)
This study evaluates, for the first time, the seasonal (winter and summer) and geographical (São Miguel⁻SMG and Santa Maria⁻SMA Islands) variability of Fucus spiralis (Fs) biochemical composition (dry weight basis) and antioxidant properties. Protein and carbohydrates presented higher values in...
Karina Jiménez-Durán et al.
Plant physiology, 161(1), 97-107 (2012-11-15)
In Solanaceae, the self-incompatibility S-RNase and S-locus F-box interactions define self-pollen recognition and rejection in an S-specific manner. This interaction triggers a cascade of events involving other gene products unlinked to the S-locus that are crucial to the self-incompatibility response....
Tadashi Nakai et al.
The Journal of biological chemistry, 287(9), 6530-6538 (2012-01-12)
Quinohemoprotein amine dehydrogenase (QHNDH), an αβγ heterotrimer present in the periplasm of several Gram-negative bacteria, catalyzes the oxidative deamination of various aliphatic amines such as n-butylamine for assimilation as carbon and energy sources. The γ subunit of mature QHNDH contains...
H Ikemura et al.
The Journal of biological chemistry, 262(16), 7859-7864 (1987-06-05)
Subtilisin E, an alkaline serine protease of Bacillus subtilis 168, is first produced as a precursor, pre-pro-subtilisin, which consists of a signal peptide for protein secretion (pre-sequence) and a peptide extension of 77 amino acid residues (pro-sequence) between the signal...
实验方案
To standardize a procedure for the enzymatic assay of Protease using Casein as a substrate.
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相关内容
建立以酪蛋白为底物的蛋白酶的标准酶法测定方法。
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