Merck
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文件

P4333

Sigma-Aldrich

青链霉素

Solution stabilized, with 10,000 units penicillin and 10 mg streptomycin/mL, 0.1 μm filtered, BioReagent, suitable for cell culture

所有图片(1)
别名:
Pen-Strep
NACRES:
NA.76

质量水平

300

无菌性

0.1 μm filtered

产品线

BioReagent

形式

liquid

浓度

100 ×

technique(s)

cell culture | mammalian: suitable

杂质

endotoxin, tested

抗生素抗菌谱

Gram-negative bacteria
Gram-positive bacteria

作用机制

cell wall synthesis | interferes
protein synthesis | interferes

运输

dry ice

储存温度

−20°C

SMILES string

CC1(C)S[C@@H]2[C@H](NC(=O)Cc3ccccc3)C(=O)N2[C@H]1C(O)=O.CNC[C@H]4[C@H](O)[C@@H](O)[C@H](CCO)O[C@H]4O[C@H]5[C@@H](O[C@@H](C)C5(O)C=O)O[C@H]6[C@H](O)[C@@H](O)[C@H](NC(N)=N)[C@@H](O)[C@@H]6NC(N)=N

InChI

1S/C23H43N7O12.C16H18N2O4S/c1-7-23(38,6-32)18(42-19-8(5-28-2)12(33)13(34)9(40-19)3-4-31)20(39-7)41-17-11(30-22(26)27)14(35)10(29-21(24)25)15(36)16(17)37;1-16(2)12(15(21)22)18-13(20)11(14(18)23-16)17-10(19)8-9-6-4-3-5-7-9/h6-20,28,31,33-38H,3-5H2,1-2H3,(H4,24,25,29)(H4,26,27,30);3-7,11-12,14H,8H2,1-2H3,(H,17,19)(H,21,22)/t7-,8-,9-,10+,11-,12-,13-,14+,15-,16+,17+,18-,19-,20-,23+;11-,12+,14-/m01/s1

InChI key

KMOOCZWLFBSQCW-XFOLRDBHSA-N

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此商品
P4458P4083P0781
Penicillin-Streptomycin Solution stabilized, with 10,000 units penicillin and 10 mg streptomycin/mL, 0.1 μm filtered, BioReagent, suitable for cell culture

Sigma-Aldrich

P4333

青链霉素

Penicillin-Streptomycin Solution Stabilized, with 5,000 units penicillin and 5mg streptomycin/mL, 0.1 μm filtered, BioReagent, suitable for cell culture

Sigma-Aldrich

P4458

青链霉素

Sigma-Aldrich

Sigma-Aldrich

P4083

Penicillin − Streptomycin − Neomycin Solution Stabilized

Penicillin-Streptomycin with 10,000 units penicillin and 10 mg streptomycin per mL in 0.9% NaCl, 0.1 μm filtered, BioReagent, suitable for cell culture

Sigma-Aldrich

P0781

青链霉素

product line

BioReagent

product line

BioReagent

product line

BioReagent

product line

BioReagent

form

liquid

form

liquid

form

liquid

form

liquid

concentration

100 ×

concentration

50 ×

concentration

-

concentration

100 ×

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

一般描述

青霉素链霉素溶液，也称为Pen Strep，是培养哺乳动物细胞最常用的抗生素溶液。青霉素链霉素溶液可用于维持细胞培养过程中的无菌条件，并且它们对培养的细胞几乎没有不良影响。

应用

青霉素-链霉素可用于补充细胞培养基以控制细菌污染。推荐以10 mL/L的浓度用于细胞培养应用。
青霉素链霉素溶液已在多种类型的培养基中用作补充剂，包括RGC（视网膜神经节细胞）培养、 α-MEM（最低必需培养基）生长培养基、 Dulbecco′s改良Eagle′s培养基（DMEM）/营养混合物F-12 Ham、 DMEM 和DMEM/ F-12 + GlutaMax。

组分

该产品配方包含10,000单位的青霉素和10 mg链霉素/mL，溶于专有的柠檬酸盐缓冲液中。

其他说明

20 ml、100 ml
保存于密闭容器内，置于干燥通风处。打开的容器必须小心地重新密封，并保持直立以防止泄漏。储存等级（TRGS 510）：不可燃液体

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(F)

Not applicable

闪点(C)

Not applicable

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Dulbecco’s Modified Eagle’s Medium - high glucose With 4500 mg/L glucose, L-glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

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D5796

培养基

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L-Glutamine–Penicillin–Streptomycin solution

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MEM 非必需氨基酸溶液 (100×)

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胰蛋白酶-EDTA 溶液

Sodium pyruvate solution 100 mM, sterile-filtered, BioReagent, suitable for cell culture

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丙酮酸钠 溶液

RPMI-1640 Medium With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

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RPMI-1640 培养基

ZhiMin Wang et al.
Transplantation, 103(3), 502-511 (2018-09-14)
Advances in immunosuppressive therapy have significantly improved short-term but not long-term survival of cardiac transplant recipients; this is largely due to severe cardiac allograft vasculopathy (CAV). Glucagon-like peptide-1 receptor (GLP-1R)-based therapy exerts physiological effects on the cardiovascular system in addition
184: Toxic effects of antibiotics routinely used in cell culture on primary cultures of dissociated mouse brain cells.
Ammon F
Pediatric Research, 10, 900-900 (1976)
J H Martínez-Liarte et al.
Pigment cell research, 8(2), 83-88 (1995-04-01)
Penicillin and streptomycin, the most widely used antibiotics in mammalian cell cultures, caused a moderate stimulation in dopa oxidase and tyrosine hydroxylase activities, but a slight inactivation in the dopachrome tautomerase activity of B16/F10 melanoma cells at the routine concentration
David Sabourin et al.
Journal of laboratory automation, 18(3), 212-228 (2012-09-28)
A microfluidic component library for building systems driving parallel or serial microfluidic-based assays is presented. The components are a miniaturized eight-channel peristaltic pump, an eight-channel valve, sample-to-waste liquid management, and interconnections. The library of components was tested by constructing various
Ian M Evans et al.
Molecular and cellular biology, 31(6), 1174-1185 (2011-01-20)
Neuropilin-1 (NRP1) is a receptor for vascular endothelial growth factor (VEGF) and plays an important role in mediating cell motility. However, the NRP1 signaling pathways important for cell motility are poorly understood. Here we report that p130(Cas) tyrosine phosphorylation is
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