Merck
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P4417

Sigma-Aldrich

磷酸盐缓冲盐水

tablet

别名:
PBS
MDL编号:
eCl@ss:
32129211
NACRES:
NA.25

形式

tablet

质量水平

200

pH值(酸碱度)

7.2-7.6 (1 tablet/200 mL)

溶解性

water: soluble

application(s)

diagnostic assay manufacturing

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相关类别

一般描述

磷酸盐缓冲液(PBS)是一种用于生物学研究的缓冲溶液。它是一种含有磷酸钠、氯化钠并在某些配方中含有氯化钾和磷酸钾的水基盐溶液。溶液的重量摩尔渗透压浓度和离子浓度与人体相同(等渗),并且对绝大多数细胞无毒。

应用

磷酸缓冲盐已用于-
  • 生产铂纳米颗粒(NP-Pt)水解胶体
  • 在SEM之前清洗血液样品和SEM(扫描电子显微镜)存根
  • 制备荧光标记小麦胚芽凝集素(WGA)溶液
  • 在ELISA(酶联免疫吸附测定)期间稀释捕获抗体
  • 在离心过程中悬浮细胞沉淀
  • 免疫测定程序

包装

50, 100 tablets in poly bottle

重悬

将一片溶于200ml去离子水中,在25 °C下得到0.01m磷酸盐缓冲液、0.0027m氯化钾和0.137m氯化钠,pH值为7.4。

储存分类代码

13 - Non Combustible Solids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

分析证书

请输入批号搜索分析证书(COA)。

原产地证书 (CofO)

请输入批号搜索原产地证书(COO)。

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. Does Product P4417, Phosphate buffered saline (PBS), contain calcium or magnesium?

    No.  The complete formulation is given in the product listing.  No calcium or magnesium salts are included in the preparation.

  4. Does one tablet of Product P4417, Phosphate buffered saline (PBS), make up 1X or 10X PBS?

    When 1 tablet is reconstituted in 200 ml of water, the resulting solution is 1X PBS.

  5. Can Product P4417, Phosphate buffered saline (PBS), solution be autoclaved?

    Yes.  PBS is not harmed by standard autoclave procedures.

  6. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  7. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

M Michaelis et al.
The journal of physical chemistry. B, 123(31), 6694-6704 (2019-07-11)
Although successful in the structural determination of ordered biomolecules, the spectroscopic investigation of oligopeptides in solution is hindered by their complex and rapidly changing conformational ensemble. The measured circular dichroism (CD) spectrum of an oligopeptide is an ensemble average over
Lipid rafts in the bovine milk fat globule membrane revealed by the lateral segregation of phospholipids and heterogeneous distribution of glycoproteins.
Lopez C et al
Food Chemistry, 120.1, 22-33 (2010)
Structural damage of chicken red blood cells exposed to platinum nanoparticles and cisplatin.
Kutwin M et al
Nanoscale Research Letters, 9(1), 257-257 (2014)
Simon C Drew et al.
PloS one, 5(12), e15875-e15875 (2011-01-07)
The amyloid-β (Aβ) peptide is the primary component of the extracellular senile plaques characteristic of Alzheimer's disease (AD). The metals hypothesis implicates redox-active copper ions in the pathogenesis of AD and the Cu(2+) coordination of various Aβ peptides has been
Wenwen Yu et al.
Food chemistry, 241, 493-501 (2017-09-30)
The conversion of barley starch to sugars is a complex enzymic process. Most previous work concerned the biotechnical aspect of in situ barley enzymes. However, the interactions among the macromolecular substrates and their effects on enzymic catalysis has been little

实验方案

Antibody Purification using Protein A, Protein G, or Protein L Agarose

Antibody Purification using Protein A, Protein G, or Protein L Agarose protocol is designed as a quick purification method for antibodies from mammalian sera, ascites, and cell culture supernatants. It should be noted that if the starting material is serum or ascites the final preparation will contain endogenous host IgG as well as specific antibodies. In general, the presence of this endogenous IgG should not interfere with assays using the antibodies.

Immunofluorescence Labeling of Cells

Cell staining can be divided into four steps: cell preparation, fixation, application of antibody, and evaluation.

ELISA Procedures

ELISA protocols providing detailed instructions on performing indirect ELISA and Capture ELISA . Learn more about recommended products and techniques for performing both sandwich ELISA and indirect ELISA experiments.

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

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ELISA 操作步骤

ELISA实验方案提供了进行间接ELISA和捕获ELISA的详细说明。了解进行夹心式ELISA和间接ELISA实验的推荐产品和技术信息。

免疫组织化学操作步骤

本实验方案通过对石蜡包埋的组织切片中的特定抗原进行染色和成像,完成整个免疫组织化学(IHC)操作步骤。

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