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应用
碱性磷酸酶可与抗体和其他蛋白质偶联,用于ELISA、蛋白质免疫印迹和组织化学检测。 碱性磷酸酶也可用于防止DNA自身连接和放射性标记。
生化/生理作用
碱性磷酸酶是一种水解酶,能使核苷酸、蛋白质和生物碱等分子去磷酸化。
包装
包装规格取决于DEA单位
单位定义
在pH 9.8和37℃条件下,1.0个单元每分钟水解1 μmol对硝基苯基磷酸盐。
外形
从Tris-HCl缓冲液中冻干,含氯化镁和氯化锌,pH 8.0
分析说明
通过缩二脲法测定的蛋白质。
底物
警示用语:
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
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Sequencing end-labeled DNA with base-specific chemical cleavages.
A M Maxam et al.
Methods in enzymology, 65(1), 499-560 (1980-01-01)
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实验方案
碱性磷酸酶的酶法测定,二乙醇胺法(EC 3.1.3.1)
Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)
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