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P4439

Sigma-Aldrich

碱性磷酸酶 来源于猪肾脏

lyophilized powder, ≥100 DEA units/mg protein

别名:
Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
CAS号:
EC 号:
MDL编号:
eCl@ss:
42010105

形式

lyophilized powder

质量水平

200

specific activity

≥100 DEA units/mg protein

组成

Protein, ≥50%

储存温度

−20°C

应用

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. Alkaline phosphatase is also used to prevent DNA self ligation and for radiolabeling.

包装

100 units in serum bottle
Package size based on DEA units

生化/生理作用

Alkaline phosphatase is a hydrolase enzyme that dephosphorylates molecules such as nucleotides, proteins and alkaloids.

单位定义

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 9.8 at 37 °C.

外形

Lyophilized fromTris-HCl buffer, with magnesium chloride and zinc chloride, pH 8.0

分析说明

Protein determined by biuret.

储存分类代码

13 - Non Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

分析证书

原产地证书 (CofO)

Sequencing end-labeled DNA with base-specific chemical cleavages.
A M Maxam et al.
Methods in enzymology, 65(1), 499-560 (1980-01-01)
Lars Hansen et al.
American journal of human genetics, 92(4), 575-583 (2013-04-09)
PGAP2 encodes a protein involved in remodeling the glycosylphosphatidylinositol (GPI) anchor in the Golgi apparatus. After synthesis in the endoplasmic reticulum (ER), GPI anchors are transferred to the proteins and are remodeled while transported through the Golgi to the cell
Insulin affects dopamine metabolism in Drosophila females under normal and stress conditions.
N V Adonyeva et al.
Doklady. Biochemistry and biophysics, 448, 40-42 (2013-03-13)
Yuan-Kun Zhai et al.
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica, 38(1), 105-111 (2013-04-20)
To investigate the effects of naringin on the proliferation, differention and maturaion of rat calvarial osteoblasts (ROB). Segregated neonatal SD rat skull, enzyme digestion to obtain ROB. The culture medium was replaced every three days. Serial subcultivation proceeded when cells
J A Otegbayo et al.
African journal of medicine and medical sciences, 41(4), 417-422 (2013-05-16)
HAART associated hepatoxicity is an important cause of poor adherence to therapy in HIV infected persons. An initial manifestation is elevation in the level ofAlanine Transaminase (ALT) in blood. We sought to evaluate the protective effects of Livolin, a phosphatidylcholine

实验方案

Diethanolamine Assay for the Enzymatic Assay of Alkaline Phosphatase (EC 3.1.3.1)

Enzymatic Assay of Alkaline Phosphatase, Diethanolamine Assay (EC 3. 1. 3. 1)

相关内容

二乙醇胺测定法用于碱性磷酸酶的酶法测定(EC 3.1.3.1)

碱性磷酸酶的酶法测定,二乙醇胺法(EC 3.1.3.1)

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