P4860

Sigma-Aldrich

蛋白酶 来源于地衣芽孢杆菌

≥2.4 U/g

别名:
Alcalase® 2.4L, Subtilisin Carslberg, 蛋白酶 来源于地衣芽孢杆菌, Subtilisin A, 枯草杆菌蛋白酶A
CAS号:
EC 号:
MDL编号:
eCl@ss:
32160410
NACRES:
NA.54

质量水平

200

形式

aqueous solution

specific activity

≥2.4 U/g

储存温度

2-8°C

正在寻找类似产品? Visit 产品对比指南

相关类别

一般描述

蛋白水解酶具有催化、非催化和辅助结构域。蛋白水解酶大致分为内肽酶和外肽酶。根据功能可分为天冬氨酸、谷氨酸、半胱氨酸、苏氨酸、丝氨酸和金属蛋白酶。

应用

Sigma 公司的酶已被用于制备鸡(腿)骨蛋白水解产物,同时评估 ACE 抑制肽。在研究它们的抗肿瘤活性时,它还被用于水解蛋白核小球藻(Chlorella pyrenoidosa)蛋白质提取物。
蛋白酶是一种通过水解肽键来分解蛋白的酶。 蛋白酶用于降解蛋白质,研究蛋白酶抑制剂和研究热失活动力学。 蛋白酶用于孵育中的核酸分离步骤。

生化/生理作用

蛋白酶通过肽键的水解使蛋白质分解代谢。 蛋白酶被丝氨酸活性位点抑制剂灭活,例如苯甲基磺酰氟(PMSF)和二异丙基氟磷酸盐。
碱性蛋白酶是丝氨酸型的内切蛋白酶。它具有广泛的底物特异性,可以水解蛋白质分子中的大多数肽键。它在 pH 6.5 和 8.5 之间有活性,最适温度为 60°C。该酶可用在洗涤剂配方中,以去除蛋白质污渍。

分析说明

用安森单位表示的活性

法律信息

Novozyme公司的产品
Alcalase is a registered trademark of Novozymes Corp.

警示用语:

Danger

hazcat

Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

storage_class_code

10 - Combustible liquids

WGK Germany

WGK 1

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

分析证书

原产地证书 (CofO)

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. What is the composition of the solution in which P4860 Protease from Bacillus licheniformis is prepared?

    The composition of P4860 is 50% (w/w) glycerol, 41% (w/w) water, and 9% (w/w) enzyme.

  6. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Fu-Yuan Cheng et al.
Animal science journal = Nihon chikusan Gakkaiho, 80(1), 91-97 (2010-02-19)
This study aims to identify peptides with angiotensin-I converting enzyme (ACE) inhibitory activity in hydrolysate from chicken leg bone protein hydrolyzed with alcalase for 4 h (A4H). The hydrolysate has demonstrated potent in vitro ACE inhibitory activity, and has been...
Xiaoqin Wang et al.
Biotechnology progress, 29(3), 681-687 (2013-04-23)
Chlorella pyrenoidosa is a unicellular green algae and has been a popular foodstuff worldwide. However, no reports on the antitumor peptides from such a microalgae are available in the literature. In this study, using low-temperature high-pressure extraction, enzymatic hydrolysis, ion...
Proteases: multifunctional enzymes in life and disease
Lopez-Otin C and Bond JS
Test, 283(45), 30433-30437 (2008)
Catherine Cesa-Luna et al.
PloS one, 15(10), e0240545-e0240545 (2020-10-16)
The bacterial strain, EMM-1, was isolated from the rhizosphere of red maize ("Rojo Criollo") and identified as Pseudomonas protegens EMM-1 based on phylogenetic analysis of 16S rDNA, rpoB, rpoD, and gyrB gene sequences. We uncovered genes involved in the production...
Ghanendra Gartaula et al.
Carbohydrate polymers, 196, 199-208 (2018-06-13)
The cell walls of cereal endosperms are a major source of fibre in many diets and of importance in seed structure and germination. Cell walls were isolated from both pure wheat endosperm and milled flour. 13C CP/MAS NMR in conjunction...

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门