P5380

Sigma-Aldrich

蛋白酶 来源于地衣芽孢杆菌

Type VIII, lyophilized powder, 7-15 units/mg solid

别名:
Subtilisin Carslberg, 蛋白酶 来源于地衣芽孢杆菌, Subtilisin A, 枯草杆菌蛋白酶A
CAS号:
EC 号:
MDL编号:
NACRES:
NA.54

质量水平

200

类型

Type VIII

形式

lyophilized powder

specific activity

7-15 units/mg solid

分子量

27 kDa

纯化方式

crystallization

溶解性

10 mM NaAc (pH 7.5) and 5 mM CaAc: soluble, clear
H2O: soluble, colorless

异质活性

DNase ≤5.0 Kunitz units/mg solid
RNase ≤0.05 Kunitz units/mg solid

储存温度

−20°C

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相关类别

一般描述

该酶在pH 8-10下稳定,可在24小时保存高达90%的活性。其55-60℃下具有最大活性。

特异性

枯草素A 是丝氨酸S8内蛋白酶家族的一个成员。它具有广泛的特异性,在P1位置有较大的非荷电残基。它可水解天然和变性蛋白,并且在碱性条件下具有活性。

应用

这是由 衣芽孢杆菌发酵而分离出来的一种蛋白水解酶。这是一种丝氨酸内蛋白酶,对天然和变性蛋白具有广泛的特异性,在碱性条件下具有活性。
该产品已与其他酶一起用于 原位 蛋白水解,以制备适用于结构测定的晶体。它还被用于分离可用于 体外 功能研究的肌膜下(SS)和肌原纤维间(IMF)线粒体。

包装

25 mg in poly bottle
1 g in glass bottle
100, 250 mg in glass bottle

生化/生理作用

蛋白酶通过水解肽键分解蛋白质。 蛋白酶可被丝氨酸活性位点抑制剂灭活,如苯基甲基磺酰氟(PMSF)和二异丙基氟磷酸酯。 蛋白酶,P5380的产物,是一种丝氨酸内蛋白酶,对天然和变性蛋白具有广泛的特异性,在碱性条件下具有活性。 它在某些有机溶剂中具有活性,如干辛烷。

物理属性

枯草素 是一种无二硫键的非糖基化单肽链,其分子量为27kda。

单位定义

一单位在37°C、pH 7.5条件下每分钟可水解1.0μmole(181 μg)的酪氨酸(福林酚试剂显色)。

警示用语:

Danger

hazcat

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

storage_class_code

10 - Combustible liquids

WGK Germany

WGK 1

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

分析证书

原产地证书 (CofO)

L Proctor et al.
Gynecologic oncology, 146(3), 596-602 (2017-06-26)
We sought to determine whether DNA ploidy correlates with the four molecular subgroups of endometrial carcinoma (EC) as determined using ProMisE (Proactive Molecular Risk Classifier for Endometrial Cancer). 90 cases of EC previously characterized by clinicopathological parameters, outcomes, and ProMisE...
Christoph Schiklenk et al.
The Journal of cell biology, 217(7), 2383-2401 (2018-05-08)
Although the formation of rod-shaped chromosomes is vital for the correct segregation of eukaryotic genomes during cell divisions, the molecular mechanisms that control the chromosome condensation process have remained largely unknown. Here, we identify the C2H2 zinc-finger transcription factor Zas1...
Carley R Benton et al.
The Journal of physiology, 586(6), 1755-1766 (2008-02-02)
Peroxisome proliferator-activated receptors (PPARs) alter the expression of genes involved in regulating lipid metabolism. Rosiglitazone, a PPARgamma agonist, induces tissue-specific effects on lipid metabolism; however, its mode of action in skeletal muscle remains unclear. Since fatty acid translocase (FAT/CD36) was...
Tommi Kotila et al.
Nature communications, 9(1), 1892-1892 (2018-05-16)
Actin polymerization powers key cellular processes, including motility, morphogenesis, and endocytosis. The actin turnover cycle depends critically on "re-charging" of ADP-actin monomers with ATP, but whether this reaction requires dedicated proteins in cells, and the underlying mechanism, have remained elusive....
Akanksha Thawani et al.
Nature cell biology, 20(5), 575-585 (2018-04-27)
How microtubules (MTs) are generated in the cell is a major question in understanding how the cytoskeleton is assembled. For several decades, γ-tubulin has been accepted as the universal MT nucleator of the cell. Although there is evidence that γ-tubulin...
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