Merck
All Photos(1)

P6282

Sigma-Aldrich

聚-L-赖氨酸 氢溴酸盐

mol wt 70,000-150,000, lyophilized powder, γ-irradiated, BioXtra, suitable for cell culture

Synonym(s):
PLL HBr
CAS Number:
MDL编号:
eCl@ss:
32160406
PubChem化学物质编号:
NACRES:
NA.75

质量水平

200

无菌性

γ-irradiated

产品线

BioXtra

形式

lyophilized powder

分子量

70,000-150,000

包装

pkg of 5 mg

technique(s)

cell culture | mammalian: suitable

溶解性

H2O: soluble 50 mL/vial, clear, colorless

运输

ambient

储存温度

−20°C

SMILES string

Cl.NCCCCC(N)C(O)=O

InChI

1S/C18H38N6O4/c19-10-4-1-7-13(22)16(25)23-14(8-2-5-11-20)17(26)24-15(18(27)28)9-3-6-12-21/h13-15H,1-12,19-22H2,(H,23,25)(H,24,26)(H,27,28)/t13-,14-,15-/m0/s1

InChI key

WBSCNDJQPKSPII-KKUMJFAQSA-N

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应用

聚-L-赖氨酸氢溴酸盐已用于预涂体外细胞培养用玻璃底器皿。
当使用0.5 ml的0.1 mg/ml溶液覆盖25cm2时,推荐其作为细胞培养基质。 必须针对每种细胞系和应用确定最佳的贴壁条件。

组分

聚-L-赖氨酸是一种带正电荷的氨基酸聚合物,每个赖氨酸残基约有一个HBr。 氢溴酸盐可使聚-L-赖氨酸呈可溶于水的结晶形式。 在β结构中可能会发现少量的产物,因为HBr干扰氨基与羧基或含N或O部分之间的氢键。

注意

无菌溶液可在2-8℃条件下稳定保存2年。脱水保存在-20°C条件下。

分析说明

分子量基于粘度。同样通过 MALLS 分析。

其他说明

有关聚氨基酸的其他技术信息,请访问 聚氨基酸常见问题解答资源

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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Articles

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Protocols

Poly-L-Lysine Cell Attachment Protocol

Adhere cells to solid substrates using poly-lysine, which enhances electrostatic interaction between negatively charged ions of the cell membrane and the culture surface.

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多聚-L-赖氨酸细胞附着实验方案

在生物应用中,聚氨基酸可以帮助细胞和蛋白质附着在固体表面。在细胞培养中,多种细胞的正常附着、生长和发育都取决于附着因子和细胞外基质成分。

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