所有图片(1)

P6486

Millipore

EZview 红色蛋白 A 亲和凝胶

MDL编号:
NACRES:
NA.56

形式

liquid

质量水平

200

analyte chemical class(es)

proteins (Immunoglobulins of various mammalian species)

应用

affinity chromatography: suitable
immunoprecipitation (IP): suitable

容量

~4 mg binding capacity (rabbit IgG)

运输

wet ice

储存温度

−20°C

一般描述

EZview Red Protein A亲和凝胶是一种易于观察的红色蛋白A亲和凝胶。亲和树脂含有通过共价键连接到交联4%琼脂糖珠上的金黄色葡萄球菌蛋白A。该亲和凝胶用于将抗体IgG 的FC部分与样品结合,如细胞裂解物和组织。结合EZview Red Protein A亲和凝胶的免疫复合物通过离心回收。红色染料增强了可视性,以获得更有效的结果。

应用

EZview Red Protein A Affinity亲和凝胶已被用于纯化免疫沉淀和和染色质免疫沉淀(ChIP) 检测中的蛋白质。
当进行小规模的亲和力捕获(例如免疫沉淀)时,亲和基质很难在微量离心管中看到。 意外吸入树脂会导致结果的定量变化。 EZview红色亲和凝胶大大降低了沉淀损失的风险。 EZview树脂的性能与传统的非彩色亲和凝胶一样,但使得用户可以轻松区分沉淀物与上清液。这与更准确的数据相关,因为损失的蛋白质更少。

包装

1 mL in poly bottle

特点和优势

  • 可见性增加-红色可降低意外吸入的风险
  • 通过减少意外损失提高目标蛋白的回收率
  • 重现性更高-收率更一致

外形

50% slurry in PBS with 50%Glycerol and 0.0015%
含有50%甘油和15 ppm Kathon的PBS的1:1 (v/v)悬液。

储存及稳定性

EZview Red Protein A亲和凝胶应当在-20 °C下在50%甘油中储存。按规定储存时本品可以在至少一年内保持稳定。 不可在没有甘油的情况下冷冻。

法律信息

EZview is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

分析证书

原产地证书 (CofO)

NF-?B directly regulates ?-arrestin-1 expression and forms a negative feedback circuit in TNF-a?induced cell death
Li J, et al.
Faseb Journal (2018)
James Matsunaga et al.
Microbiology (Reading, England), 152(Pt 12), 3777-3786 (2006-12-13)
Leptospirosis is a widespread zoonosis caused by invasive spirochaetes belonging to the genus Leptospira. Pathogenic leptospires disseminate via the bloodstream to colonize the renal tubules of reservoir hosts. Little is known about leptospiral outer-membrane proteins expressed during the dissemination stage...
Katriina Kahlos et al.
Molecular and cellular biochemistry, 254(1-2), 47-54 (2003-12-17)
We previously reported that exposure to exogenous nitric oxide (NO) causes diminished expression of thioredoxin/thioredoxin reductase, a critical component of the redox system that regulates the functions of redox-sensitive enzymes, receptors, and transcription factors. Here we examined the role of...
A laboratory practical illustrating the use of the ChIP-qPCR method in a robust model: Estrogen receptor alpha immunoprecipitation using Mcf-7 culture cells
Lacazette E
BioChemistry: An Indian Journal (2017)
Hsp90 Co-chaperone p23 contributes to dopaminergic mitochondrial stress via stabilization of PHD2: Implications for Parkinson's disease.
Rane A, et al.
Neurotoxicology (2018)

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