P6887

Sigma-Aldrich

胃蛋白酶 来源于猪胃粘膜

lyophilized powder, ≥3,200 units/mg protein

别名:
胃液素 来源于猪胃, 胃蛋白酶 A
CAS号:
EC 号:
MDL编号:
eCl@ss:
42010127
NACRES:
NA.54

质量水平

300

形式

lyophilized powder

specific activity

≥3,200 units/mg protein

分子量

35 kDa

杂质

salt, essentially free

颜色

white to off-white

溶解性

deionized water: soluble 10 mg/mL
10 mM HCl: soluble 4.0 mg/mL (Cold)

特色行业

Diagnostic Assay Manufacturing

UniProt登记号

运输

wet ice

储存温度

−20°C

Gene Information

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相关类别

应用

Sigma的胃蛋白酶已与其它酶一起用于面包中抗酶淀粉(RS)的测定。它也被用来模拟豌豆或乳清蛋白分离物的 体外 胃肠消化。
胃蛋白酶是一种消化酶,用于消化蛋白质,通常用于从抗体中制备Fab片段。从猪胃粘膜中提取的胃蛋白酶已可用于水解小鼠的干性宫颈样本。 产品P6887为冻干粉,用于在膳食纤维分析中消化蛋白质。
胃蛋白酶切割可用于产生抗体的F(ab′)2 片段。有关胃蛋白酶的更多信息,请访问 www.sigma-aldrich.com/enzymeexplorer

包装

250 mg in glass bottle
1, 5, 10 g in glass bottle

生化/生理作用

与许多其他肽酶不同,胃蛋白酶只水解肽键,而不水解酰胺或酯键。其裂解特异性包括肽键两侧含有芳香酸的肽段,特别是当另一残基也是芳香或二羧酸时。如果肽键附近有含硫氨基酸,即含有芳香氨基酸,则易水解。胃蛋白酶也会优先在苯基丙氨酸和亮氨酸的羧基侧裂解,在谷氨酸残基的羧基侧裂解的程度较小。它不裂解缬氨酸,丙氨酸,或甘氨酸键。Z-L-酪氨酸、Z-L-谷氨酰胺-L-酪氨酸或Z-L-蛋氨酸-L-酪氨酸可作为胃蛋白酶消化的底物。胃蛋白酶被几种含有苯丙氨酸的肽所抑制。
优先裂解:P1 和 P1′ 位的疏水性和芳族残基。降解胰岛素 β 链中的 Phe-Val、Gln-His、Glu-Ala、Ala-Leu、Leu-Tyr、Tyr-Leu、Gly-Phe、Phe-Phe 和 Phe-Tyr 键

单位定义

使用血红蛋白为底物的三氯乙酸(TCA)可溶性产物测量,一个单位在 37°C pH 2.0下每分钟产生 0.001 的ΔA280 。(最终体积= 16 ml。光靖= 1 cm。)

分析说明

最适pH值为2-4。在4 M尿素和3 M盐酸胍中可保持活性。在60°C时稳定。胃蛋白酶在pH 8.0-8.5下发生不可逆灭活。
蛋白质由E1%/280测定

其他说明

请于 www.sigma-aldrich.com/enzymeexplorer了解 胃蛋白酶 的更多信息。

象形图

Exclamation markHealth hazard

警示用语:

Danger

hazcat

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

storage_class_code

11 - Combustible Solids

WGK Germany

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

分析证书

原产地证书 (CofO)

V Vermeirssen et al.
Journal of dairy science, 86(2), 429-438 (2003-03-22)
Pea and whey protein were fermented by Lactobacillus helveticus and Saccharomyces cerevisiae in monoculture and in combination at 28 and 37 degrees C in order to release angiotensin-I-converting enzyme (ACE) inhibitory peptides. The fermentation products were subjected to in vitro...
Enzyme-resistant starch. III. The quality of straight-dough bread containing varying levels of enzyme-resistant starch.
Eerlingen, R. C., et al.
Cereal Chem., 71(2), 165-169 (1994)
Kakoli Das et al.
Cancer letters, 353(2), 167-175 (2014-08-03)
Gastric cancer (GC) is a major cause of global cancer mortality. Previous genomic studies have reported that several RTK-RAS pathway components are amplified in GC, with individual tumours often amplifying one component and not others ("mutual exclusivity"). Here, we sought...
Konstantinos Korompokis et al.
Food & function, 10(8), 4674-4684 (2019-07-12)
Wheat grain roller milling disrupts starch containing endosperm cell walls and extracts white flour. Many wheat based food processes involve simultaneous use of heat and water which then cause starch to gelatinize and enhance its digestibility. In this study, the...
J A Marlett et al.
The American journal of clinical nutrition, 50(3), 479-485 (1989-09-01)
The effects of different methods on the distribution of total neutral sugars (TNS), uronic acids (UA), and beta-glucans (beta G) between the soluble (S) and insoluble (I) fractions of dietary fiber (DF) were determined for peas, kidney beans, oat bran...
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