P6998

Protein Kinase A Catalytic Subunit β, Active human

recombinant, expressed in baculovirus infected Sf9 cells, ≥80% (SDS-PAGE), buffered aqueous glycerol solution

• Synonym(s): PKA Catalytic Subunit β, Active human, PKA-Cβ), cAMP-dependent protein kinase
• Enzyme Commission number: 2.7.11.11 (BRENDA, IUBMB)
• MDL编号: MFCD07371063

Properties

• 重组: expressed in baculovirus infected Sf9 cells
• 质量水平: 200
• 检测方案: ≥80% (SDS-PAGE)
• 形式: buffered aqueous glycerol solution
• specific activity: ≥10 nmol/min·mg
• 分子量: protein apparent mol wt ~65 kDa
• UniProt登记号: P22694
• 运输: dry ice
• 储存温度: −70°C
• Gene Information: human ... PRKACB(5567)

Description

一般描述

Protein kinase cAMP-activated catalytic subunit β (PRKACB) is one of the catalytic subunits of the serine/threonine kinase, protein kinase A. The gene encoding it is localized on human chromosome 1p36.1.

应用

Kinase activity is measured as the molar amount of phosphate incorporated into the CREBtide substrate peptide per minute per mg protein at 30 ^°C using a final concentration of 50 μM [³²P] ATP.

生化/生理作用

cAMP-dependent protein kinase (PKA; EC 2.7.1.37) is an essential enzyme in the signaling pathway of the second messenger cAMP. Through phosphorylation of target proteins, PKA controls many biochemical events in the cell including regulation of metabolism, ion transport, and gene transcription.

单位定义

One unit will incorporate one nanomole of phosphate into the Crebtide (KRREILSRRPSYR) substrate per minute at 30 deg C and pH 7.2 using a final concentration of 50 μM [32P] ATP.

外形

Supplied as a solution of 5 μg in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF and 25% glycerol.

Safety Information

• 储存分类代码: 12 - Non Combustible Liquids
• WGK: WGK 1
• 闪点(F): Not applicable
• 闪点(C): Not applicable